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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (21): 88-91.doi: 10.11924/j.issn.1000-6850.2012-3571

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

一株琼胶酶产生菌的筛选与鉴定

缪伏荣   

  • 收稿日期:2012-11-02 修回日期:2012-12-14 出版日期:2013-07-25 发布日期:2013-07-25
  • 基金资助:
    环境友好型饲料关键技术集成及其产业化开发

Screening and Molecular Identification of a New Agarase—producing Bacterium

  • Received:2012-11-02 Revised:2012-12-14 Online:2013-07-25 Published:2013-07-25

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摘要： 利用琼胶作唯一碳源,从腐烂的紫菜中分离筛选到1株高酶活力的琼胶降解细菌Z705。对其菌落形态、生理生化性质和酶活进行了初步研究，并克隆其16S rDNA序列,进行分子鉴定。结果表明：该菌株为革兰氏阴性，呈短杆状；与模式菌株嗜麦寡养食单胞菌Stenotrophomonas maltophilia（AB008509）的同源性为99%；根据表型特征、生理生化特性和分子鉴定将菌株Z705鉴定为寡养单胞菌属（Stenotrophomonas sp.）。

关键词: 环境质量评价, 环境质量评价

Abstract: Agar as the sole carbon source, to separation the screening plant height enzyme Agarolytic bacteria Z705 from rotting seaweed. The colony morphology, physiological and biochemical properties and enzyme activity was studied, and the clone its 16 s rDNA sequence, molecular identification.The results show that Z705 is gram negative, a short rod; strain Z705 displayed a high sequence similarity with Stenotrophomonas maltophilia (99%). According to its morphological, physiological and biochemical properties and the homology analysis of 16S rDNA sequence, strain Z705 was identified as Stenotrophomonas sp.