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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (16): 69-73.doi: 10.11924/j.issn.1000-6850.2012-3670

所属专题： 烟草种植与生产

• 林学 园艺 园林 • 上一篇    下一篇

镉胁迫下不同品种烟草镉化学形态分布研究

李彦娥 赵秀兰   

  • 收稿日期:2012-11-12 修回日期:2013-01-10 出版日期:2013-06-05 发布日期:2013-06-05

Chemical Forms of Cadmium in the Different Tobacco Cultivars Under Cd Stress

  • Received:2012-11-12 Revised:2013-01-10 Online:2013-06-05 Published:2013-06-05

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摘要： 为研究镉胁迫下不同品种烟草积累和忍耐镉的差异及机制问题，以烟草品种‘RG17’、‘贵烟11号’、‘Xanthi’为试验材料，采用土培试验方法对不同品种烟草镉化学形态分布特征进行了研究。结果表明，烟草根、茎、叶镉的各形态含量随土壤镉浓度的增加而增加，根部FHAC分配比率最高，占绝对优势，茎FHAC降低，FNaCl增加，而叶片FNaCl分配比率最高，占绝对优势，且随着镉浓度的加大，根、茎、叶镉的各形态分配比率有接近的趋势。同样各形态分配比率因品种而异，根部‘Xanthi’FHAC分配比率最高，而FW、FNaCl分配比率低；叶内FNaCl分配比率‘Xanthi’高于其他品种，而FW分配比率低，随着镉浓度的增加，这种趋势更加明显。推断高积累型烟草根内镉形成难溶于水的磷酸盐有利于其对镉的积累，而与蛋白质络合，从细胞质转移到液泡可能是烟草耐性品种忍耐镉的主要机制之一。

关键词: TCA-Sevag法, TCA-Sevag法

Abstract: Pot experiment was carried out to test the differences of Cd chemical forms among different tobacco Cultivars. The Cd forms in plants shows that the FHAC proportion is highest in root, FHAC proportion decreased and FNaCl increased in stem, while tha FNaCl proportion becomes highest in leaf, which shoed the different mechanisms of cadmium tolerance in the roots, stems and leaves of tobacco. With the increase of Cd in soil, the content of every form of Cd also increased. The FHAC proportion in root of high accumulator is higher than thatof low accumulators and the FW, FNacl proportion is lower than thatof low accumulators. The FNacl proportion in leaf of tolerance accumulator is higher and Fw of tolerante accumulator is lower than that of sensitive accumulators. It implies that the complex of cadmium and protein might be the reason why some tobacco cultivars are tolerant to cadmium. the complex of cadmium and protein is transported from cytoplasm to vacuole, which is compartmented in the vacuole.