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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (29): 42-45.doi: 10.11924/j.issn.1000-6850.2012-3780

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

外源性孕酮对牛卵母细胞体外核成熟的影响

杨菲 姚学军 闫芃 梁琳 郑雪莹 邢书涵 刘云海 郭勇 倪和民   

  • 收稿日期:2012-11-21 修回日期:2013-01-01 出版日期:2013-10-15 发布日期:2013-10-15
  • 基金资助:
    2011年度国家自然基金面上项目“不同来源植入前期绵羊胚胎IFNτ差异表达及其体外诱导子宫上皮细胞Galectin15;2013年度国家自然基金面上项目“牛体内外植入前胚胎IFNτ的差异表达及对子宫

The Effect of Exogenous Progesterone on in vitro Nuclear Maturation of Bovine Oocytes

  • Received:2012-11-21 Revised:2013-01-01 Online:2013-10-15 Published:2013-10-15

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摘要： 了研究外源性孕酮对牛卵母细胞体外核成熟的作用，通过向成熟培养液中添加10 μg/mL FSH +10 μg/mL LH（对照组）和0 ng/mL、10 ng/mL、100 ng/mL、1000 ng/mL的外源性孕酮，对牛卵丘卵母细胞复合体进行体外成熟培养，在培养8 h后用醋酸地衣红染色观察各自的生发泡破裂率，然后在成熟22 h统计第一极体率，体外受精后7~8天统计囊胚率。结果表明，对照组卵母细胞的生发泡破裂率(75.0%)和极体率(79.6%)均明显高于添加不同浓度孕酮的各处理组(P<0.05)，而囊胚率各组之间均无显著差异(P>0.05)。因此，外源性孕酮不能促进牛卵母细胞的核成熟，单独添加外源性孕酮不能起到与内源性孕酮相同的作用。

关键词: 农业生产效率, 农业生产效率

Abstract: In this experiment, it was aimed to investigate the effect of exogenous progesterone on in vitro nuclear maturation of bovine oocytes through adding with 10 μg/mL FSH + 10 μg/mL LH (control), 0 ng/mL, 10 ng/mL, 100 ng/mL, and 1000 ng/mL exogenous progesterone respectively into the in vitro maturation medium for bovine cumulus-oocyte complexes (COCs). The germinal vesicle breakdown (GVBD) rate of each group after 8 h using acetic acid-orcein staining, and counted first polarbody (PbI) rate after 22 h were all analyzed. Finally, the blastocyst rate after 7-8 days of in vitro fertilization was also counted. The results showed that, GVBD (75.0%) and PbI (79.6%) rate of the control group were significantly higher than the other ones adding with exogenous progesterone (P<0.05), and blastocyst rate of each group didn’t show significant variation (P>0.05). Therefore, it could be concluded that the exogenous progesterone could not play a role for improving the nuclear maturation of bovine oocytes, it also meant that exogenous progesterone alone could not take the same effect as endogenous progesterone in such kind of processes.