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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (23): 15-20.doi: 10.11924/j.issn.1000-6850.2012-3885

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

养殖池塘脱氮硫杆菌(Thiobacillus denitrificans)的空间分布及与相关理化因子关系研究

裘丽萍 范立民 王可赟 陈家长   

  • 收稿日期:2012-11-30 修回日期:2013-02-28 出版日期:2013-08-15 发布日期:2013-08-15
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项资金项目;现代农业产业技术体系建设专项资金项目

Spatial Distribution of Thiobacillus denitrificans in Intensive Pond System and Its Relationship with Some Related Physicochemical Indicators

  • Received:2012-11-30 Revised:2013-02-28 Online:2013-08-15 Published:2013-08-15

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摘要： 为了研究养殖池塘中脱氮硫杆菌的分布状况及在水体中与相关理化因子的关系，分别分层采集吉富罗非鱼(Oreochromis niloticus)精养池塘底泥样品和水样，采用最大可然数法(MPN)对底泥和水体中脱氮硫杆菌进行定量分析，同时测定分层水样理化指标值。结果表明：底泥和水体中，随着深度增加，脱氮硫杆菌数量均逐渐减少，底泥中变化为从每克干重105数量级降到102数量级，水体中为从每毫升103数量级降到102数量级；脱氮硫杆菌数量与几种理化因子间相关性分析结果表明，其与硝态氮呈正相关关系，与硫化物呈负相关关系，原因可能为硫化物和硝酸根的分布为池塘中溶氧分布必然结果，而硝酸根诱导了其细胞内同化型硝酸还原酶活性。

关键词: 冷沙黄泥, 冷沙黄泥

Abstract: In order to investigate the spatial distribution of Thiobacillus denitrificans in the intensive pond system and the relationship between the number of Thiobacillus denitrificans and some physicochemical indicators in the water body, columnar sediments samples and stratified water samples water were collected, and quantitative analysis of Thiobacillus denitrificans in the samples were done with the method of most probable number (MPN), at the same time, such physicochemical indicators in the water samples as the pH, the redox potential, the sulfide, the nitrate nitrogen and the nitrite nitrogen were analyzed. The results showed that: the numbers of Thiobacillus denitrificans in the sediments and water samples all decreased with the depth increased. For the sediments, the numbers decreased from 1.4×105 strains per gram (dry weight) to 9×102 strains per gram (dry weight). And for the water samples, the numbers decreased from 4.5×103 strains per milliliter to 1.4×102 strains per milliliter. And through correlation analysis, a positive correlation between the numbers of Thiobacillus denitrificans and the nitrate nitrogen, and a negative correlation between the numbers of Thiobacillus denitrificans and the sulfide were found, the reason for which might be that the spatial distribution of the nitrate and the sulfide were strongly affected by the distribution of dissolved oxygen and the nitrate induced the assimilative nitrate reductase in the cell.

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