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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (21): 170-174.doi: 10.11924/j.issn.1000-6850.2013-0240

• 植物保护 农药 • 上一篇    下一篇

环介导恒温扩增技术（LAMP）及其在植物病毒检测中的应用

秦文韬 王忠跃 张昊   

  • 收稿日期:2013-01-23 修回日期:2013-03-03 出版日期:2013-07-25 发布日期:2013-07-25
  • 基金资助:
    农业部公益性行业（农业）科研专项

Loop-mediated isothermal amplification（LAMP）and its application in detection of plant virus

  • Received:2013-01-23 Revised:2013-03-03 Online:2013-07-25 Published:2013-07-25

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摘要： 摘要：环介导恒温扩增技术是一种特异、灵敏、简单的新型核酸扩增技术，目前，该技术已经被广泛应用于疾病诊断、食品安全检测及基因芯片等领域。本文综述了环介导恒温扩增法在植物病毒、类病毒检测中的应用，并讨论了其在应用中出现的问题及解决办法。

关键词: 超氧化物歧化酶（SOD）, 超氧化物歧化酶（SOD）

Abstract: Abstract：Loop-mediated isothermal amplification（LAMP） is a new nucleic acid amplification technology with the advantage of high specificity, efficiency and rapidity. At present, the technology has been widely used in disease diagnosis, food safety testing and gene chip, etc. This paper reviews application of LAMP in the detection of plant virus and viroid, then discusss the problems and solutions which discovered in the process of application.