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中国农学通报 ›› 2013, Vol. 29 ›› Issue (32): 87-90.doi: 10.11924/j.issn.1000-6850.2013-0441

• 水产 渔业 • 上一篇    下一篇

中药复方A对施氏鲟抗氧化能力的影响

王荻 刘红柏   

  • 收稿日期:2013-02-19 修回日期:2013-03-06 出版日期:2013-11-15 发布日期:2013-11-15
  • 基金资助:
    冷水性鱼类病害防治技术研究;冷水性鱼类主要流行性疾病中药防治技术研究

Effects of Prescription A on Anti-oxidation Capability in Amur sturgeon (Acipenser schrencki Brandt.)

Wang Di, Liu Hongbai   

  • Received:2013-02-19 Revised:2013-03-06 Online:2013-11-15 Published:2013-11-15

摘要: 为了研究自组中药方剂(复方A)对施氏鲟组织中抗氧化功能相关的活性物质含量及酶活性的影响及其在高温应激条件下对鲟鱼的免疫调节功能。将1+龄实验鱼随机分为2组,每组设3个平行,每个平行20尾鱼,采用口灌水煎剂的方法,按照1.1 g/kg体质量的剂量每日口灌给药一次,连续给药7天,并设口灌蒸馏水的对照组。实验结束后,取部分鱼高温30℃热刺激2 h后与常温22℃给药的实验组共同采样,对鱼血浆、肝脏及红细胞中SOD活性;血浆及肝脏中MDA含量;血浆及肝脏中NO含量和NOS活性进行了测定。结果表明:复方A能有效调节鲟鱼体内NO含量及NOS活性,显著提高机体SOD活性,并降低MDA含量;在高温应激条件下仍能保持良好的作用效果,保护机体免受氧化损伤。复方A是一种适用于鲟鱼养殖使用的免疫添加剂,在高温应激条件下仍有较好效果。

关键词: 瓦房店, 瓦房店

Abstract: In order to research the enzyme activities and contents of active materials, which were involved in anti-oxidation capability of tissues, were investigated in Amur sturgeon (Acipenser schrencki Brandt.) when oral given the prescription of Traditional Chinese Medicine A. The immune function of prescription A on sturgeons was also studied under high temperature stress condition. 1+ age old fish were divided into 2 groups each (n=60 in each group), with each group subdivide into 3 replicates of 20 fish per tank. A control group, in which distilled water was administered orally, was also tested. Prescription A was orally given to experimental fish at a concentration of 1.1 g/kg body weight continuously for 7 days. After the administration of drugs, parts of fish were stimulated for 2 hours at high temperature (30℃) and samples were collected. Superoxide Dismutase (SOD) activity in plasma, liver and red blood cells, Malondialdehyde (MDA) and Nitric Oxide (NO) content in plasma and liver, and Nitric Oxide Synthase (NOS) activity in plasma and liver, were measured and compared. The results indicate that prescription A could efficiently regulate NO content and NOS activity, increase SOD activity and decrease MDA content in several tissues of Amur sturgeon. The prescription A also brought better effects to protect the body from oxidative damage under the thermal stimulation. Thus, the prescription A was a suitable Traditional Chinese Medicine for sturgeon farming as an immune additive since it still had fine effect under the high temperature stress condition.

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