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中国农学通报 ›› 2013, Vol. 29 ›› Issue (31): 118-122.doi: 10.11924/j.issn.1000-6850.2013-0658

• 林学 园艺 园林 • 上一篇    下一篇

欧李增殖培养基的优化研究

孙晓丽 贾艳芳 高仪 王红霞 张志华   

  • 收稿日期:2013-03-11 修回日期:2013-05-20 出版日期:2013-11-05 发布日期:2013-11-05

The optimized research on proliferation culture medium of Chinese Dwarf Cherry

  • Received:2013-03-11 Revised:2013-05-20 Online:2013-11-05 Published:2013-11-05

摘要: 【研究目的】筛选出适合欧李增殖的最适培养基,可以提高试管苗的质量和优化欧李无性繁殖体系。【方法】以欧李2号增殖培养阶段试管苗为试材,以不同培养基、不同激素浓度、不同蔗糖浓度、不同pH值为主要研究内容,进行了增殖效果研究。【结果】MS培养基最适宜欧李2号的增殖;NAA 0.2 mg/L+6-BA 0.5 mg/L+MS处理,其平均分化新梢数和大于2 cm新梢数最多;MS培养基中蔗糖浓度40 g/L处理,既可显著提高分化新梢数又可提高成苗率;pH6.0的MS培养基最适宜欧李2号生长。【结论】适于欧李2号的增殖培养基为MS培养基+NAA 0.2 mg/L+6-BA 0.5 mg/L+6 g琼脂+40 g蔗糖,pH值为6.0。

关键词: 出茧率, 出茧率

Abstract: 【Objective】Selected the most suitable medium for Cerasus humilis proliferation could improve the quality of the plantlets and optimize asexual reproduction system of Cerasus humilis. 【Method】The proliferation culture stage plantlets of Chinese Dwarf Cherry 2 were as test materials,and different media, different hormone concentrations and different sucrose concentrations and different pH were as the main content to study the proliferation effect. 【Result】The most appropriate medium for Cerasus humilis 2 to Proliferation was MS . In the treatment of NAA 0.2 mg / L +6- BA 0.5 mg / L + MS , the average differentiated shoots number and the number of shoots greater than 2 cm were the largest. The treatment of MS medium with sucrose concentration of 40 g / L can not only significantly increase the number of differentiated shoots but also improve seedling rate. The MS medium with pH 6.0 was most suitable for the growth of Cerasus humilis 2. 【Conclusion】 Suitable for Prunus Humilis Bunge 2 proliferation medium was MS +NAA 0.2 mg/L+6-BA 0.5 mg/L+6 g agar +40 g sucrose, pH value is 6.0.

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