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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (31): 123-128.doi: 10.11924/j.issn.1000-6850.2013-0737

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

葡萄VvWRKY71转录因子的生物信息学分析

侯丽霞 王文杰 刘新   

  • 收稿日期:2013-03-18 修回日期:2013-04-23 出版日期:2013-11-05 发布日期:2013-11-05
  • 基金资助:
    葡萄抗寒相关基因克隆及其作用机制的研究;葡萄ICE基因克隆及其抗寒机制的研究;葡萄抗寒基因克隆和表达特性分析

Bioinformatics Analysis of VvWRKY71 Transcription Factor in Vitis vinifera L

  • Received:2013-03-18 Revised:2013-04-23 Online:2013-11-05 Published:2013-11-05

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摘要： WRKY转录因子在植物中广泛存在，参与了生长发育和逆境等多种生物学过程。利用生物信息学方法对葡萄VvWRKY71转录因子的理化性质、二级结构、保守结构域、亚细胞定位、功能和系统进化等方面进行了预测和分析。结果表明：该蛋白由311个氨基酸组成，分子量为34.8KDa，属于亲水性不稳定蛋白；蛋白质二级结构主要由无规则卷曲和α-螺旋组成；其C端具有高度保守的WRKY结构域，亚细胞定位结果表明该蛋白位于细胞核，功能预测结果显示其主要参与了转录和转录调控过程。

关键词: 差动电感传感器应用, 差动电感传感器应用

Abstract: The characteristic of VvWRKY71 transcription factor in grape was analyzed and predicted by bioinformatic methods in the following aspects: physical and chemical characters, secondary and tertiary structure of protein, conserved domain, subcellular localization, function category and molecular phylogenetic evolution. The results showed that VvWRKY71 was an unstable hydrophilic proteins which made of 311 amino acid with molecular weight 34.8KDa; Random coil and α-helix were the major structural element of polypeptide chain; And it possessed one conserved WRKY domain, so it belonged to WRKY transcription factor; Subcellular localization result indicated that VvWRKY71 was located in nucleus very likely. Function analysis indicated that it was involved in transcription and transcription regulation. This research showed VvWRKY71 would play important function as a transcription factor.