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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (31): 91-95.doi: 10.11924/j.issn.1000-6850.2013-0772

所属专题： 食用菌； 食用菌

• 林学 园艺 园林 • 上一篇    下一篇

杏鲍菇原生质体制备条件初探

张鹏1,2,3,龚玲凤3,4,庄卫东1,郑金水1,朱坚2,3   

  • 收稿日期:2013-03-20 修回日期:2013-04-14 出版日期:2013-11-05 发布日期:2013-11-05
  • 基金资助:
    福建省科技重大专项“动植物良种培育及关键配套技术研究与示范（食用菌）”(2008SZ0002)

Preliminary Study on Separation of Pleurotus eryngii Protoplast

Zhang Peng1,2,3, Gong Lingfeng3,4, Zhuang Weidong1, Zheng Jinshui1, Zhu Jian2,3   

  • Received:2013-03-20 Revised:2013-04-14 Online:2013-11-05 Published:2013-11-05

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摘要： 为了提高杏鲍菇原生质体产量，以杏鲍菇菌株为材料，通过单因素试验研究酶浓度、酶解时间、酶解温度、菌丝培养方式、菌龄以及稳渗剂种类对杏鲍菇原生质体制备的影响。结果表明：杏鲍菇原生质体制备的最佳条件为:酶浓度2%、酶解时间2.5 h、酶解温度29℃、菌丝液体静置培养5天，以甘露醇为稳渗剂，在此条件下原生质体产量为33.4×106个/mL，为杏鲍菇原生质体技术育种提供参考。

关键词: 鹿泉市, 鹿泉市

Abstract: The optimal conditions such as enzyme concentration、enzymatic hydrolysis time、reaction temperature、mycelium culture way、mycelium age and type of osmotic stabilizer for the lywallzyme hydrolysis of the cultured mycelia of Pleurotus eryngii for protoplast production were investigated by single factor experiment. The results showed that the highest production rate of the protoplast was emerged under the conditions that 5 days old liquid mycelium in the enzyme solution of 2% lywallzyme digested for 2.5h at temperature of 29℃ with 0.6mol/L mannitol as osmotic pressure stabilizer. This study has laid the foundation of protoplast breeding of Pleurotus eryngii in the future.

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