关键词: Western Blot, Western Blot

Abstract: The objectives of this study were to find out the molecular markers related to carcass and meat quality economic traits, and provide the theoretical basis of molecular genetics for breeding the new groups of Gansu beef cattle. PCR-SSCP was used to detect the polymorphisms of exon 2 and intron 3 of LEP gene in Gansu local cattle and analyze its correlation with carcass and meat quality traits. The results showed that AA and AB genotypes were found in the LEP gene exon 2, the genotype frequencies were 0.5217 and 0.4783. Sequence analysis showed that there existed a mutation of C→T at 1180bp of LEP gene and resulted Arginine to Cysteine. The PIC was between 0.25-0.5, it was moderate polymorphism. Statistical analysis of carcass and meat quality traits showed that AA genotype has a significant effect on before slaughter live weight, carcass weight, meat weight, dressing percentage and meat percentage (P<0.05), AB genotype has a significant effect on pressing loss, leaf fat weight, marbling score and meat color (P<0.05), this site has no significant association with backfat thickness, loin eye area, cooking loss, shear force and pH (P>0.05). The preliminary judgment of this site can be considered as one of the genetic markers loci of carcass and meat quality traits.