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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (25): 32-36.doi: 10.11924/j.issn.1000-6850.2013-0970

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

双向电泳联用质谱技术研究松材线虫和拟松材线虫的差异蛋白

罗茂春 刘颖 林静雯 王勤 潘沧桑 陈亮   

  • 收稿日期:2013-04-07 修回日期:2013-05-24 出版日期:2013-09-05 发布日期:2013-09-05

Analysis of Differential Proteins between Bursaphelenchus xylophilus and B. mucronatus Using Two Dimensional Polyacryamide Electrophoresis and Mass Spectrometry

  • Received:2013-04-07 Revised:2013-05-24 Online:2013-09-05 Published:2013-09-05

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摘要： 松材线虫是造成松树萎蔫病的病原，对松林威胁很大。其近似种拟松材线虫与松材线虫在形态学上极其相似，却不具致病性。所以，松材线虫和拟松材线虫的快速检测至关重要。应用双向电泳联用质谱技术，研究松材线虫和拟松材线虫的蛋白差异，并对差异蛋白进行MALDI-TOF/MS分析以及数据库鉴定，共鉴定了45个差异蛋白，其中松材线虫22个、拟松材线虫23个。不仅为松材线虫和拟松材线虫的准确鉴定打下基础，差异蛋白的进一步研究可望揭示松材线虫的致病机理。

关键词: 在线监控, 在线监控

Abstract: The plane-parasitic nematode Bursaphelenchus xylophilus is the causal agent of pine wilt disease, which is the most serious conifer disease in the world. However, it is difficult to differentiate isolates of B. xylophilus from the closely related species B. mucronatus, which is not pathogenic to pine trees. So it is very important to distinguish B. mucronatus from B. xylophilus rapidly. We applied the two-dimensional electrophoresis and mass spectrometry to study the proteomics of the two nematodes. And we have obtained 45 differentiate proteins, 22 of which were from B. xylophilus and others were from B. mucronatus. The results supply new insights into the rapid identification of B. xylophilus the virulence of B. xylophilus.