欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (28): 60-65.doi: 10.11924/j.issn.1000-6850.2013-1357

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

红叶乌桕组织培养技术研究

王春荣 张往祥 毕君 曹福亮   

  • 收稿日期:2013-05-13 修回日期:2013-06-14 出版日期:2013-10-05 发布日期:2013-10-05
  • 基金资助:
    逆境生态林树木种质优选与示范

Study on Rapid Micropropagation System of Euphorbia cotinifolia by Tissue Culture

Wang Chunrong   

  • Received:2013-05-13 Revised:2013-06-14 Online:2013-10-05 Published:2013-10-05

PDF (PC)

825

摘要： 为了实现红叶乌桕优良种源和变异个体的种质保存和快速繁殖，以带腋芽茎段、茎尖、叶片为外植体，进行了红叶乌桕的组织培养技术研究。结果表明：6月中旬取半木质化茎段，用0.1%HgCl2灭菌5min，外植体接种成功率达79.5%；MS+KT0.5mg/L+IBA0.5 mg/L培养基适于试管苗继代增殖，20~25d继代一次长势良好；试管苗在MS+IBA0.5mg/L+KT(0.5~1.0)mg/L和1/2MS+IBA (0.1~0.5)mg/L培养基中生根效果较好，生根率在91.14%以上，平均主根数为6.49~13.03条/株。

关键词: 新品种培育, 新品种培育

Abstract: For germplasm conservation and rapid propagation of Euphorbia cotinifolia, the stem-segment with axillary buds，stem tips and leaflet taken as explants to be studied for tissue culture system. The results showed that, the optimizing sterilization method for stem-segment with axillary buds in the second ten days of June was that explants could be treated with 0.1% HgCl2 for 5 min. The percentage of sterile explant could arrive at 79.5%. It was suggested that MS+KT0.5mg/L+IBA0.5 mg/L to be desirable treatment for subculture and multiplication, with 2.45 times multiplication rate after a 20-25 days cultivation. The media with MS+IBA0.5mg/L+KT(0.5~1.0)mg/L and 1/2MS+IBA (0.1~0.5)mg/L could be used for the rooting induction . The rooting rate was 91.14% . The average number of rootlet for the test-tube plantlets was 6.49~13.03.