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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (35): 53-57.doi: 10.11924/j.issn.1000-6850.2013-1567

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

多功能纤维素酶(EGXA)基因在福寿螺胃、肠和肝脏表达水平的测定

程天印 李姣   

  • 收稿日期:2013-06-05 修回日期:2013-07-29 出版日期:2013-12-15 发布日期:2013-12-15
  • 基金资助:
    入侵生物福寿螺的防控

On the Expressions Level of EGXA Gene in the Stomach, Intestines, Hepatopancreas of Pomacea canaliculata

  • Received:2013-06-05 Revised:2013-07-29 Online:2013-12-15 Published:2013-12-15

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摘要： 为了探明多功能纤维素酶在不同消化器官的表达情况，采用RT-PCR方法测定了多功能纤维素酶在野生福寿螺胃、肠和肝脏3种组织中的相对表达量。结果表明：EGXA基因在胃、肠和肝脏的表达水平(2-⊿⊿Ct)依次为：1.56±0.13、0.08±0.01、0.12±0.03，即多功能纤维素酶主要由福寿螺胃分泌。

关键词: 小鼠生物检测法, 小鼠生物检测法

Abstract: To investigate the expression of EGXA (the multi-functional enzyme with the activities of exo-β-1,4-glucanase, endo-β-1,4-glucanase and endo-β-1,4-xylanase) gene in different digestive organs of Pomacea canaliculata, the expression amount of the EGXA gene in the snail's stomach, intestine, hepatopancreas was evaluated by real-time fluorescent quantitative PCR technique. The results showed that the expression levels (2-⊿⊿Ct) of EGXA gene in the snail's stomach, intestine, hepatopancreas were 1.56±0.13, 0.08±0.01, 0.12±0.03 respectively. It suggested that the EGXA was secreted by the stomach of Pomacea canaliculata chiefly.