大白菜eIF(iso)4E.a假基因突变体的鉴别及相关检测标记的开发

doi:10.11924/j.issn.1000-6850.2013-1607

中国农学通报 ›› 2014, Vol. 30 ›› Issue (4): 101-107.doi: 10.11924/j.issn.1000-6850.2013-1607

所属专题：生物技术；园艺

大白菜eIF(iso)4E.a假基因突变体的鉴别及相关检测标记的开发

刘栓桃张志刚李巧云王淑芬卢金东张晓燕徐文玲刘贤娴付卫民赵智中

收稿日期:2013-06-08 修回日期:2013-07-22 出版日期:2014-02-05 发布日期:2014-02-05
基金资助:
山东省科技发展计划“大白菜抗芜菁花叶病毒位点特异分子标记开发体系构建”(2012GNC11209)；国家“十二五”科技支撑计划“十字花科蔬菜杂种优势利用与新品种选育”(2012BAD02B01-6)；山东省现代农业产业技术体系“山东省现代蔬菜产业技术体系”(SDNYKJCX-2013-02)。

Characterization of Mutation at Locus eIF(iso)4E.a and Development of Related Markers in Chinese Cabbage

Received:2013-06-08 Revised:2013-07-22 Online:2014-02-05 Published:2014-02-05

摘要/Abstract

摘要： 马铃薯Y病毒在侵染寄主时与eIF4E或eIF(iso)4E的相互作用是一种普遍机制。大白菜病毒病主要病源是TuMV，TuMV在侵染大白菜时能同时与eIF4E和eIF(iso)4E发生作用，且在大白菜基因组中上述两基因各有三个拷贝。因而鉴定大白菜资源在上述两个位点的多样性、发现各位点的功能缺失突变体至关重要。采用同源克隆和Tail-PCR技术，鉴定了12份大白菜抗/感TuMV材料中eIF(iso)4E.a位点的多样性。结果表明：5份材料的eIF(iso)4E.a基因组序列完整，但与已知序列相比均存在显著差异，包含3种新的单倍型；7份材料的eIF(iso)4E.a缺失了第四、五外显子及3'端约1 kb的大片段，是个假基因。同时开发了鉴别完整基因与假基因的共显性标记。该策略为鉴定大白菜中eIF4E和eIF(iso)4E其他位点的多样性提供了借鉴，开发的相关检测标记可以作为标记辅助选择的工具用于创新抗病毒大白菜新种质及培育抗病毒大白菜新品种。

关键词: 除草活性, 除草活性

Abstract: Interaction between virus of Potyviridae family and eIF4E and eIF(iso)4E is a common mechanism when they infect plant. TuMV of Potyviridae family is the most serious virus resource infecting Chinese cabbage. It can use both eIF4E and eIF(iso)4E when infection and each gene has three copies in genome of Chinese cabbage. Thus it’s necessary to screen the status of each locus among core Chinese cabbage germplasms resistant or susceptible to TuMV. At present study, we characterized three novel haplotypes among five lines possessing intact eIF(iso)4E.a gene; the status of eIF(iso)4E.a pseudogene was identified by Tail-PCR among seven lines studied. A set of codominant markers were developed to distinguish the intact state and pseudogene mutation. The stratage used in the study can still be used to identify status of other loci of eIF4E and eIF(iso)4E family or other functional genes. The markers could be used for breeding new virus-resistant varieties through polymerized cross and marker assisted selection when mutations and markers of other loci were identified.

刘栓桃张志刚李巧云王淑芬卢金东张晓燕徐文玲刘贤娴付卫民赵智中. 大白菜eIF(iso)4E.a假基因突变体的鉴别及相关检测标记的开发[J]. 中国农学通报, 2014, 30(4): 101-107.

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大白菜eIF(iso)4E.a假基因突变体的鉴别及相关检测标记的开发

Characterization of Mutation at Locus eIF(iso)4E.a and Development of Related Markers in Chinese Cabbage

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