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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (4): 113-119.doi: 10.11924/j.issn.1000-6850.2013-1668

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

基于AFLP和SSR分子标记的苹果属植物亲缘关系研究

李佳 石琰璟 沙广利 黄粤 马荣群 孙吉禄   

  • 收稿日期:2013-06-17 修回日期:2013-07-15 出版日期:2014-02-05 发布日期:2014-02-05
  • 基金资助:
    国家现代苹果产业技术体系“青岛综合试验站建设”(CARS-28)；农业公益性行业科研专项“主要果树砧木收集、评价与筛选”(201203075-03)；山东省农业良种产业化工程专项资金资助“苹果新品种和抗性砧木选育”[鲁农良种字(2010)6]。

The Genetic Relationship of Malus Species Based on AFLP and SSR Markets Analysis

  • Received:2013-06-17 Revised:2013-07-15 Online:2014-02-05 Published:2014-02-05

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摘要： 研究比较AFLP与SSR 2种分子标记技术在苹果亲缘关系上的准确性。应用AFLP、SSR、AFLP与SSR结合这3种方法，对41份苹果属内种质资源类型进行亲缘关系分析。从64对AFLP引物中筛选出7对多态性较好的引物，7对引物平均多态率为86.72%。从40对SSR引物中筛选出7对多态性较好的引物，多态率均为100.00%。UPGMA聚类结果显示，3种方法分别将41份供试材料分为6大类群，大部分种质的分类结果与传统的分类方法一致。通过聚类分析也明确了部分新发现类型的分类地位归属，合理解释了新类型的起源，也通过分析比较了3种方法的准确性，认为AFLP的聚类结果更符合传统分类及田间形态，为苹果属植物种质资源的分类方法提供了分子生物学依据。

关键词: 桃花岭村, 桃花岭村

Abstract: AFLPs and SSRs were compared in terms of their efficiency in studying the genetic relationship of 41 Malus species. Genetic relationship among 41 Malus species was analyzed by AFLP, SSR technique and AFLP+SSR. 7 primer pairs were selected from 64 AFLP primer pairs, and 7 primer pairs selected from 40 SSR primer pairs. The average polymorphic rate of 7 pairs of primers was 86.72% and 100.00%. UPGMA clustering analysis indicated that the 41 Malus species could be divided into 6 categories separately using the three methods, which were similar to the traditional classification methods. Some new found apple species were identified and clarified, the accuracy of the three methods were compared. The cluster results showed AFLP technique had higher consistency with traditional classification and field performance than SSR and AFLP+ SSR. The results provided molecular basis for classification of Malus germplasm resources.