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中国农学通报 ›› 2014, Vol. 30 ›› Issue (7): 126-129.doi: 10.11924/j.issn.1000-6850.2013-1844

• 林学 园艺 园林 • 上一篇    下一篇

‘卓锦’万代兰花梗诱导植株再生的研究

李杰 匡萍 张银洁   

  • 收稿日期:2013-07-05 修回日期:2013-07-18 出版日期:2014-03-05 发布日期:2014-03-05
  • 基金资助:
    四川省科学技术带头人培养项目“名贵兰花引种繁育及生物技术产业化集成与示范”(11sd3105);西南科技大学博士基金项目“兰花种质资源的引种和繁育”(09zx7107)。

Plantlets Regeneration from Floral Stalk of Vanda ‘Miss Joaquim’

  • Received:2013-07-05 Revised:2013-07-18 Online:2014-03-05 Published:2014-03-05

摘要: 为建立‘卓锦’万代兰的组培快繁体系,研究植物外源激素和基本培养基对‘卓锦’万代兰花梗腋芽诱导、丛生芽增殖和生根的影响。结果表明:以花梗为外植体,在1/2MS+ NAA 0.5 mg/L+ BA 2.0 mg/L培养基上,腋芽诱导率可达65%,以花梗基部向上第3节花梗腋芽诱导率最高,可达80%;在6-BA 3.0 mg/L + NAA 0.2 mg/L的激素组合下,芽的繁殖系数最高可达5.3,低无机盐浓度且含全量元素的1/2MS基本培养基较利于‘卓锦’万代兰芽的诱导增殖;丛生芽高度为2 cm左右时,壮苗生根后移栽成活率可达99%。

关键词: 直径分布, 直径分布

Abstract: In order to establish rapid propagation system of Vanda ‘Miss Joaquim’ ,effects of exogenous hormone and media on plantlets regeneration were studied. The results showed that: Decontamiated-explants can be obtained by inducing from floral stalk,and the sixty-five percent of plantlets formation ratio can be achieved on 1/2MS media supplemented with NAA0.5mg/L+6-BA2.0mg/L.There were different formation in different location in flora stalk buds and the toppest plantlets formation ratio were reached 80% in the third flora stalk buds;The propagation ratio was reached 5.3 on 1/2MS media supplemented with NAA0.2 mg/L+6-BA3.0 mg/L and the media with low concentration nutrient elements was benefit to the plantlets forming;With the length of 1~2cm, plantlets can be transplant into 1/2MS media to reduce roots ,and the percent s of survivor can be reached 99% when plantlets with roots were planted into greenhouse .