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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (1): 42-47.doi: 10.11924/j.issn.1000-6850.2013-2352

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

花曲柳愈伤组织悬浮培养及秦皮甲素积累研究

刘孚婧 詹亚光   

  • 收稿日期:2013-09-03 修回日期:2013-09-11 出版日期:2014-01-05 发布日期:2014-01-05
  • 基金资助:
    国家科技支撑计划项目 “白桦水曲柳新品种选育技术研究” (2012BAD01B0505)。

Research on callus suspension culture and the accumulation of Aesculin in Fraxinus rhynchophylla

Liu Fujing1, Zhan Yaguang1,2   

  • Received:2013-09-03 Revised:2013-09-11 Online:2014-01-05 Published:2014-01-05

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摘要： 为建立花曲柳的愈伤组织诱导、增殖与悬浮培养体系，采用WPM为基本培养基，选取休眠芽刚萌发的叶片及叶柄为外植体，采用均匀试验设计研究不同浓度及配比的生长调节剂对花曲柳愈伤组织的形成和增殖的影响。1.8 mg/L NAA和0.1 mg/L TDZ的生长调节剂组合适合形成绿色、松散的愈伤组织，1.2 mg/L NAA和0.07 mg/L TDZ的组合适合形成增殖迅速的悬浮愈伤组织团。花曲柳愈伤组织诱导的最佳培养基为WPM+1.5 mg/L NAA+ 0.1 mg/L TDZ；增殖的最佳培养基为WPM+ 1.874 mg/L NAA+ 0.127 mg/L TDZ；愈伤组织悬浮培养基为1/2WPM+ 1.2 mg/L NAA+ 0.07 mg/L TDZ，秦皮甲素最大积累量出现在悬浮培养的第9天，最高可达0.800 mg/g。

关键词: 抗病机理, 抗病机理

Abstract: The author built the system of callus induction, proliferation and suspension culture in Fraxinus rhynchophylla. Leaves and petioles germinated from dormant buds of Fraxinus rhynchophylla were chosen as explants using the WPM as the basic culture medium. The effect on the formation and proliferation of the callus regulated by plant auxin of different concentrations and proportions was determined by uniform experimental design. Calluses induced by the combination based on 1.8 mg/L NAA and 0.1 mg/L TDZ were green and loose. 1.2 mg/L NAA with 0.07 mg/L TDZ can be used to form suspended callus cultures with high proliferation rate. The callus inducing medium: WPM+1.5 mg/L NAA+ 0.1 mg/L TDZ. The best medium for callus proliferation: WPM + 1.874 mg/L NAA + 0.127 mg/L TDZ. The best medium for callus suspension culture: 1/2WPM + 1.2 mg/L NAA+ 0.07 mg/L TDZ. The maximum content of aesculin was 0.800 mg/g and it appeared on the 9th day after suspension culture.