欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (15): 310-315.doi: 10.11924/j.issn.1000-6850.2013-2394

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

米曲霉HDF-7固定化菌体发酵产蛋白酶条件的优化

周晓杭 柴洋洋 田雪 葛菁萍 平文祥   

  • 收稿日期:2013-09-09 修回日期:2013-10-16 出版日期:2014-05-25 发布日期:2014-05-25
  • 基金资助:
    国家自然科学基金“菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制”(31270534)；黑龙江大学高层次人才（创新团队）支持计划“功能微生物选育及其产物应用”(Hdtd2010-17)；黑龙江省科技厅“农业微生物发酵技术”科技创新团队。

Optimization of Fermentation Conditions for Producing Protease by Immobilized Apergillus oryzae HDF-7

ping jingge   

  • Received:2013-09-09 Revised:2013-10-16 Online:2014-05-25 Published:2014-05-25

PDF (PC)

561

摘要： 为了在工业化生产豆酱的过程中，缩短发酵周期，提高原料利用率，并降低生产成本，保证产品质量，利用从传统豆酱中分离得到的米曲霉(Aspergilleas oryzae)HDF-7作为出发菌株，经过海藻酸钠包埋法固定后，对其最佳固定化菌球的发酵产蛋白酶条件进行了优化。结果表明，固定化菌球以3%的接种量接入到中性蛋白酶发酵培养液中增殖至168 h后，其蛋白酶酶活力最高，为18.49±0.65 U/mL，利用该增殖菌球单批次发酵到24 h时，其蛋白酶活力表现最高。并可以重复分批发酵7次而蛋白酶活力保持不变。本研究通过固定化米曲霉(Aspergilleas oryzae)HDF-7菌体并优化其发酵生产蛋白酶的条件，可以缩短发酵时间，提高菌体的利用效率，并保持酶活稳定。该研究为蛋白酶的规模化生产提供了试验基础。

关键词: 鲁西南, 鲁西南

Abstract: In order to improve protease production and reduce fermentation period in the process of industrial production of bean paste, we adopt the method of the sodium alginate embedding method to immobilize Aspergilleas oryzae HDF-7, which was isolated from traditional fermented soybean as a starting strain and ascertain the best fermentation condition. Protease activity was as index, we determined that the most optimal inoculums size was 3%; the best proliferated time was 168 h; the most optimal fermented time was 24 h, it's protease activity was 18.49±0.65 U/mL. On the basis of immobilization, repeated batches of fermentation were 7 times. This study has improved the protease enzyme activity, shortened the fermentation time and increased the protease production by immobilized Apergillus oryzae HDF-7 and optimized immobilized conditions. It would settle base for the industrial production.