欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (10): 42-45.doi: 10.11924/j.issn.1000-6850.2013-2719

• 林学 园艺 园林 • 上一篇    下一篇

舞毒蛾ISSR反应体系的优化与建立

陈芳 骆有庆 石娟 邬颖 杜秋洋   

  • 收稿日期:2013-10-17 修回日期:2013-12-12 出版日期:2014-04-05 发布日期:2014-04-05
  • 基金资助:
    中央高校基本科研业务费专项“生态林重大虫灾监测与防控”(TD2011-06))；国家自然科学基金面上项目(31170613)；教育部新世纪优秀人才支持计划(NCET－10－0232)。

Optimization and Establishment of ISSR Reaction System for Gypsy Moth (Lymantria dispar Linnaeus)

  • Received:2013-10-17 Revised:2013-12-12 Online:2014-04-05 Published:2014-04-05

PDF (PC)

667

摘要： 舞毒蛾是一种食性广、危害大的林业害虫，遗传多样性的研究有利于揭示不同地理种群的遗传变异情况。以河北舞毒蛾为实验材料，对ISSR反应体系中的5个因子（Taq DNA聚合酶、模板DNA、引物、MgCl2和dNTPs）的8个浓度梯度依次进行试验，每一因子的最佳浓度都用于下一组优化试验，最终确定最佳反应体系优化筛选，结果表明，20μL ISSR反应体系各组分的最适浓度分别为0.5 U Taq DNA聚合酶，1.5 ng/μL模板DNA，2.4μmol/L引物，1.75 mmol/L MgCl2，0.28 mmol/L dNTPs。这一优化的舞毒蛾ISSR-PCR反应体系为进一步利用ISSR分子标记技术对舞毒蛾的遗传多样性分析奠定了良好的试验基础。

关键词: 烟台苹果, 烟台苹果

Abstract: The gypsy moth, Lymantria dispar Linnaeus (Lepidoptera: Lymantriidae), is a destructive defoliator with a broad host range. Studying genetic variation is a helpful tool for revealing the genetic differentiation of different geographic populations of gypsy moth. 5 main factors of ISSR reaction system for gypsy moth (Lymantria dispar Linnaeus) was optimized with sample from Hebei Province, China. Factors with 8 concentration gradients were performed successively. Each optimum concentration of a factor was applied to the next optimization experiment. The final ISSR reaction system for gypsy moth was as follows: 0.5 U Taq DNA polymerase, template DNA 1.5 ng/μL, primer 2.4μmol/L, 1.75 mmol/L MgCl2, 0.28 mmol/L dNTPs. Then ddH2O was added to the final volume of 20μL. This optimum reaction system would lay fundamental basis for the genetic diversity and variation research for gypsy moth.