高效液相色谱-蒸发光散射检测器测定中华常春藤中常春藤苷C和α-长春藤皂苷

doi:10.11924/j.issn.1000-6850.2013-2751

中国农学通报 ›› 2014, Vol. 30 ›› Issue (13): 190-193.doi: 10.11924/j.issn.1000-6850.2013-2751

高效液相色谱-蒸发光散射检测器测定中华常春藤中常春藤苷C和α-长春藤皂苷

李银花李娟黄建安李佳银张盛

收稿日期:2013-10-22 修回日期:2013-11-14 出版日期:2014-05-05 发布日期:2014-05-05
基金资助:
作物种质创新与资源利用重点实验室科学基金 “茶树中甲基化 EGCG相关基因的全长 cDNA克隆与功能研究” (12KFXM12)。

Simultaneous Determination of Hederacoside C and α-Hederin in Hedera nepalensis var. sinensis by High Performance Liquid Chromatography-Evaporative Light-Scattering Detector

Received:2013-10-22 Revised:2013-11-14 Online:2014-05-05 Published:2014-05-05

摘要/Abstract

摘要： 为了提高常春藤皂苷检测的灵敏度，以中华常春藤为试验材料，采用HPLC-ELSD对中华常春藤中常春藤苷C和α-长春藤皂苷的分析方法进行了研究。采用的色谱条件为：Welchrom C18 (250 mm×4.6 mm，5 μm)色谱柱；水-乙腈溶液为流动相，梯度洗脱；检测器为蒸发光散射检测器；流速1.0 mL/min；柱温30℃。常春藤苷C的进样量在0.19188~2.3985 μg范围内峰面积的对数与进样量的对数之间线性关系良好；α-常春藤皂苷的进样量在0.15615~4.6844 μg范围内峰面积的对数与进样量的对数之间线性关系良好。该方法灵敏度高，具有良好的稳定性和准确度。

关键词: 性别, 性别

Abstract: In order to improve the detection sensitivity of saponins, a kind of high performance liquid chromatography(HPLC) coupled with evaporative light scattering detector(ELSD) was established for the determination of hederacoside C and α-hederin in Hedera nepalensis var. sinensis. A welchrom column (250 mm×4.6 mm, 5 μm) was used with the mobile phase of water and acetonitrile solution. The flow rate was at 1.0 mL/min. This method showed good linearity for both hederacoside C (ranging from 0.19188 to 2.3985 μg) and α-hederin (ranging from 0.15615 to 4.6844 μg). The results demonstrated that this method was precise, accurate and can be used for the determination of hederacoside C and α-hederin .

李银花李娟黄建安李佳银张盛. 高效液相色谱-蒸发光散射检测器测定中华常春藤中常春藤苷C和α-长春藤皂苷[J]. 中国农学通报, 2014, 30(13): 190-193.

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高效液相色谱-蒸发光散射检测器测定中华常春藤中常春藤苷C和α-长春藤皂苷

Simultaneous Determination of Hederacoside C and α-Hederin in Hedera nepalensis var. sinensis by High Performance Liquid Chromatography-Evaporative Light-Scattering Detector

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