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中国农学通报 ›› 2014, Vol. 30 ›› Issue (16): 62-66.doi: 10.11924/j.issn.1000-6850.2013-2955

• 林学 园艺 园林 • 上一篇    下一篇

土壤水分对沙冬青幼苗生长及其光合荧光参数的影响

何芳兰 韩生慧 尉秋实 张进虎 李得禄   

  • 收稿日期:2013-11-11 修回日期:2014-01-08 出版日期:2014-06-05 发布日期:2014-06-05
  • 基金资助:
    甘肃省自然基金“民勤绿洲边缘雨养固沙林的防护效益”(1010RJZA170);国家自然基金项目“石羊河下游衰败人工固沙林中红砂定居过程及其驱动机理研究”(31360203);甘肃省自然基金“红砂在民勤绿洲边缘固定沙丘上定居过程研究(1308RJYA081)。

Effects of the Soil Moisture on Growth and Photosynthetic Fluorescence Parameters of Ammopiptanthus Seedlings

  • Received:2013-11-11 Revised:2014-01-08 Online:2014-06-05 Published:2014-06-05

摘要: 为阐明土壤水分胁迫下沙冬青幼苗形态和生理响应,以8个月苗龄的沙冬青幼苗为试材,对其在不同土壤水分条件下(土壤含水率分别为1.90%~2.19%、3.40%~3.59%、5.40%~5.59%、8.20%~8.39%)培养3个月后,借助游标卡尺、LI-6400XT光合仪和6400XT-40荧光叶室分别对其生长情况和光合参数进测定与分析。结果表明,土壤水分对沙冬青幼苗期的生长及其光合情况有着极大的影响。当土壤含水率低于3.40%时,1年生沙冬青实生苗生长严重受制,植株叶片开始出现大量枯黄或枯叶;随土壤水分逐渐减小,其净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、光化学量子效率(Fv/Fm)、稳态荧光参数(Fs)、光化学淬灭系数(qP)以及电子传递速率(ETR)均逐渐减小,胞间CO2摩尔浓度(Ci)先减小后增大,而非光化学淬灭系数qN和NPQ的值逐渐增大;土壤水分胁迫导致沙冬青幼苗光合作用的下降,是由气孔限制转变为非气孔限制,同时严重损伤了PSII反应中心,引起其光能原初捕捉能力和光能同化率减弱,增加了通过热辐射消耗的光能比例,最终导致其光合作用能力的减弱。

关键词: Fv/Fm, Fv/Fm

Abstract: In order to clarify morphological and physiological responses of Ammopiptanthus seedlings on soil moisture , eight-month old seedlings of Ammopiptanthus were cultivated for three months in four kinds of soil conditions along 1.90%-2.19%, 3.40%-3.59%, 5.40%-5.59% and 8.20%-8.39%. With vernier caliper, ruler, LI-6400xt photosynthetic apparatus and 6400XT-40 fluorescent leaf chamber, their growth and photosynthetic fluorescence parameters were measured and analyzed respectively. The results showed that the soil water content had a great significant effect on growth and photosynthetic fluorescence parameters of ammopiptanthus seedlings. When the soil water content was lower than 3.40%, the growth of Ammopiptanthus seedlings were seriously inhibited, and some of their leaves changed into yellow or withered. Simultaneously, with the soil water stress gradually intensified, all of the net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate(Tr) of Ammopiptanthus seedlings gradually reduced, and its intercellular CO2 molar concentration (Ci) first decreased and then increased. And it also implied that the soil water stress made the stomatal limitation turned into nonstomatal limitation, which resulted in the decease of leaf photosynthetic capacity. Furthermore, as the duress of soil moisture exacerbated, the determination of chlorophyll-fluorescence parameters revealed that the PSII maximal photochemical efficiency (Fv/Fm), the Photo-synthetic electron transport (ETR) and the photochemical quenching coefficient (qP) reduced, the steady-state fluorescence parameters (Fs) intermittent decreased, and the photochemical quenching coefficient qN and NPQ increased. In addition, the damage of PSII brought inactivation of photosynthesis center, decreased both primary capture capacity and assimilation efficiency of light energy, increased light energy consumption by means of heat, and final weakened photosynthesis capacity of Ammopiptanthus seedlings.