欢迎访问《中国农学通报》,

中国农学通报 ›› 2014, Vol. 30 ›› Issue (22): 109-113.doi: 10.11924/j.issn.1000-6850.2014-0029

• 林学 园艺 园林 • 上一篇    下一篇

竹醋对红色甜椒贮藏中活性氧代谢的影响

黄漫青 韦强 张海英 赵赢 贾永慧   

  • 收稿日期:2014-01-03 修回日期:2014-02-24 出版日期:2014-08-05 发布日期:2014-08-05
  • 基金资助:
    北京市农业局项目 “蔬菜高效配送保鲜技术试验示范” (20090301)。

Effects of Bamboo Vinegar on the Reactive Oxygen Metabolism of Red Sweet Pepper Fruits in Storage

  • Received:2014-01-03 Revised:2014-02-24 Online:2014-08-05 Published:2014-08-05

摘要: 为研究竹醋对红色甜椒在贮藏过程中的活性氧代谢的影响,以‘红苏珊’红色甜椒为实验材料,用蒸馏水将精制竹醋液稀释成500、1000和1500倍液,浸泡甜椒果柄3 min,以蒸馏水处理为对照(CK),擦干后装PE保鲜袋,于20℃储存。研究结果表明,500~1500倍竹醋液处理明显提高了红色甜椒的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性及总抗氧化能力(T-AOC),降低了超氧阴离子(O2-·)的产生速率和过氧化氢(H2O2)含量,降低了丙二醛(MDA)含量的积累和细胞膜渗透率。其中以1500倍竹醋稀释液处理的保鲜效果最好,与CK相比较,POD活性峰值提高了1.24倍,SOD活性提高了4.6%~100%,T-AOC提高了0.12%~0.72%,O2·-产生速率降低了27.22%~47.75%,H2O2含量减少了9.46%~43.42%,MDA含量减少了8.42%~39.59%,细胞膜渗透率降低了11.56%~37.34%。这说明竹醋具有较强的抗氧化性,可以减缓红色甜椒的膜脂过氧化作用,保持细胞膜的完整性,从而延缓衰老,起到一定的保鲜作用。

关键词: 对接机制, 对接机制

Abstract: Our study investigated the effects of bamboo vinegar on reactive oxygen metabolism of‘Hong susan red sweet pepper in storage process. Red sweet peppers were immersed in the bamboo vinegar solutions that diluted respectively by 500 times, 1000 times and 1500 times for 3 min, then dried. After that, red sweet peppers were put in PE bag and stored at 20℃. Red sweet peppers immersed in distilled water were used as control. After storage, we measured production rate of superoxide anion radical (O2-·), activity of superoxide dismutase(SOD) and peroxidase(POD), total antioxidative capacity(T- AOC), cell membrane permeability, contents of malondialdehyde(MDA) and hydrogen peroxide (H2O2) in red sweet pepper. The results showed that after treating with bamboo vinegar diluted by 500-1500 times, the activities of SOD, POD and T-AOC in red sweet pepper increased. And the production rate of O2-·, the content of H2O2 and MDA, and the permeability rate of cell membrane decreased. The bamboo vinegar diluted by 1500 times showed the most efficient preservation effects among these vinegar solutions, which, compared to CK, improved the peak value of POD activity by 1.24 times as well as the activity of SOD and T- AOC respectively by 4.6%- 100% and 0.12%- 0.72%. Meanwhile, the production rate of O2-·decreased by 27.22%-47.75%, the content of H2O2 and MDA reduced respectively by 9.46%-43.42% and 8.42%-39.59%, the osmosis rate of cell membrane decreased by 11.56%-37.34%. This study indicated that bamboo vinegar had strong antioxidation which could postpone the peroxidation of membrane lipid and keep the completeness of cell membrane, slowed down the aging of red sweet peppers, thus play a role in the preservation of the red sweet peppers.