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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (22): 161-164.doi: 10.11924/j.issn.1000-6850.2014-0166

• 林学 园艺 园林 • 上一篇    下一篇

新疆草原糙苏抑菌作用的研究

叶星 黄罗冬 索菲娅 卢帅 王卓 龚健   

  • 收稿日期:2014-01-16 修回日期:2014-03-09 出版日期:2014-08-05 发布日期:2014-08-05
  • 基金资助:
    国家自然基金项目“新疆孜然消化性胃溃疡有效部位的研究”(31160073)；新疆高等学校科研计划“仿天然生态培殖新疆虫草的关键技术研究”(XJEDU2010I12)；自治区科技项目计划“新疆虫草资源与仿天然生态培育关键技术开发”(201091245)。

Study on Antibacterial Activities of Pholmis pratensis from Xinjiang

  • Received:2014-01-16 Revised:2014-03-09 Online:2014-08-05 Published:2014-08-05

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摘要： 为探讨新疆草原糙苏的抑菌活性，对新疆草原糙苏最佳提取溶剂进行筛选并对其抑菌作用进行研究。采用滤纸片法和二倍稀释法研究新疆草原糙苏不同溶剂提取物抑菌效果，并对其最低抑菌浓度(MIC)和最低杀菌浓度(MBC)进行测定。结果表明：新疆草原糙苏95%乙醇和乙酸乙酯提取物有较强的抑菌效果。95%乙醇提取物对大肠杆菌的MIC为0.25 g/mL，MBC为0.25 g/mL；对金黄色葡萄球菌的MIC为0.0625 g/mL，MBC为0.125 g/mL；对变形杆菌的MIC为0.0625 g/mL，MBC为0.125 g/mL。新疆草原糙苏具有较明显的抑菌效果，不同溶剂提取物抑菌作用不同。

关键词: 生化特性, 生化特性

Abstract: To explore the antibacterial activity of Pholmis pratensis, the author screened best extraction solvent of Pholmis pratensis and studied its inhibitory effect. The filter paper method and the double dilution method were used to investigate the antibacterial activities of different Pholmis pratensis solvent extracts, and its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The results showed that 95% ethanol and ethyl acetate extract of Pholmis pratensis had strong antibacterial effect. The minimum inhibitory concentration (MIC) of 95% ethanol extract to Escherichia coli, Staphylococcus aureus and Proteus were 0.25, 0.0625, 0.0625 g/mL respectively and minimum bactericidal concentration (MBC) were 0.25, 0.125, 0.125 g/mL. Pholmis pratensis had obvious inhibitory effect and different solvent extracts had different antimicrobial effect.