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中国农学通报 ›› 2014, Vol. 30 ›› Issue (29): 133-141.doi: 10.11924/j.issn.1000-6850.2014-0363

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续随子种子油提取工艺响应面法优化及成分分析

田琴   

  1. 山西大学
  • 收稿日期:2014-02-17 修回日期:2014-02-17 接受日期:2014-04-15 出版日期:2014-10-31 发布日期:2014-10-31
  • 通讯作者: 田琴
  • 基金资助:
    山西省国际合作项目“中澳合作抗癌活性成分的分离鉴定”(2011081067)。

Optimization of Extraction Technique of Seed Oil from Euphorbia Lathyris L. Using Response Surface Methodology and Component Analysis

  • Received:2014-02-17 Revised:2014-02-17 Accepted:2014-04-15 Online:2014-10-31 Published:2014-10-31

摘要: 确定续随子种子油的最优提取工艺,并对其脂肪酸成分进行分析。通过比较索氏法、超声法、水酶法对续随子种子油的提取效率,选出最佳提取方法。在单因素试验基础上,采用Plackett-Burman 设计筛选索氏提取法中影响提取率的因素。以种子油提取率作为响应目标,对筛选出的因素通过中心复合设计(Central Composite Design)进行优化,确定索氏提取法的最优提取工艺。三种方法的最高得率分别为41.97%,36.38%,34.33%。提取溶剂、液料比、温度、时间为影响最明显的因素。确定了索氏提取法的最优提取工艺为:提取溶剂石油醚,液料比30:1(v:m),提取温度90℃,提取时间9 h。采用气相色谱-质谱法(GC-MS)分析种子油成分,共鉴定出8 种脂肪酸,占总脂肪酸含量的99%以上。其中,不饱和脂肪酸以油酸、亚油酸、α-亚麻酸和花生酸等为主,其含量达到总脂肪酸90%以上。在此工艺条件下,续随子种子油得率理论值为54.353%,实测值为54.175%,与理论值相比,相对误差为0.120%。研究结果可为续随子种子油的提取工艺提供参考,为续随子的进一步开发利用建立基础。

关键词: 排水单元, 排水单元

Abstract: The aim was to determine the optimal parameters in extraction of seed oil from Euphorbia Lathyris L. and analyze its fatty acid composition. The extraction efficiency of three extraction methods, including Soxhlet extraction, ultrasonic extraction and aqueous enzymatic extraction were compared. Based on the single factor experiment, four factors were screened and optimized by using Plackett- Burman (PB) design. Central Composite Design was applied to optimize the selected factors using the seed oil as the response value. The results showed that the average extraction efficiency were 41.97% , 36.38% and 34.33% , respectively. The effect of extraction solvent, ratio of liquid to dry material, temperature and time on the extraction efficiency of the seed oil were the most obvious. The optimal conditions of extraction were obtained as follows: extraction solvent as petroleum ether, ratio of liquid to material at 30:1, extraction temperature at 90℃, extraction time of 9 h. Finally, the fatty acids were analyzed by gas hromatography-mass spectrometry (GS/MS). A total of 8 kinds of fatty acid were identified, accounting for more than 99% of the total fatty acids. Unsaturated fatty acid included oleic acid, linoleic acid, linolenic acid, a-andarachidonic acid, and their content reached more than 90% of total fatty acids. Under these conditions the predictive maximum yield of seed oil of Euphorbia Lathyris L was 54.353% , the actual yield was 54.175% , the relative error was 0.120% . The experiment provided a reference for the extraction of oil from the seed of Euphorbia Lathyris L, and laid a foundation for future research and development of the plant.