新疆牛羊源耐药大肠杆菌β-内酰胺酶及16S rRNA甲基化酶基因检测

doi:10.11924/j.issn.1000-6850.2014-0911

中国农学通报 ›› 2014, Vol. 30 ›› Issue (35): 87-92.doi: 10.11924/j.issn.1000-6850.2014-0911

所属专题：生物技术；畜牧兽医

新疆牛羊源耐药大肠杆菌β-内酰胺酶及16S rRNA甲基化酶基因检测

底丽娜,南海辰,夏利宁,王谨

新疆农业大学动物医学学院,新疆农业大学动物医学学院,新疆农业大学,新疆农业大学

收稿日期:2014-04-01 修回日期:2014-12-14 接受日期:2014-05-27 出版日期:2015-03-18 发布日期:2015-03-18
通讯作者: 夏利宁
基金资助:
国家自然科学基金项目“新疆猪源大肠杆菌耐药基因的流行性调查及其传播机制研究”(31260614)；新疆维吾尔自治区普通高校重点学科（基础兽医学）科研启动基金。

Detection ofβ-Lactamase and 16S rRNA Methylase Gene in Resistant Escherichia coli Isolates from Cattle and Sheep in Xinjiang

Received:2014-04-01 Revised:2014-12-14 Accepted:2014-05-27 Online:2015-03-18 Published:2015-03-18

摘要/Abstract

摘要： 为了解新疆某羊场、牛场耐药大肠杆菌携带β-内酰胺酶和/或16S rRNA甲基化酶基因及其共存情况。通过PCR方法对168株羊源和32株牛源耐β-内酰胺类药物大肠杆菌进行β-内酰胺酶及16S rRNA甲基化酶基因检测，并对检出基因的PCR产物测序。检测结果为：羊源大肠杆菌携带的β-内酰胺酶基因为blaTEM(42.26%，71/168)和blaCTX-M(2.98%，5/168)；牛源大肠杆菌携带的β-内酰胺酶基因为blaTEM (15.63%，5/32)基因，未检出blaCTX-M；两个养殖场均未检出其他被检基因。羊源、牛源大肠杆菌携带的16S rRNA甲基化酶基因为rmtB基因，其检出率分别为4.17%(7/168)和50.00%(16/32)，均未检出armA基因。基因共存情况：3(1.79%)株羊源菌同时携带blaTEM和blaCTX-M基因，7（4.17%）株羊源菌同时携带blaTEM和rmtB基因；2(6.25%)株牛源菌同时携带blaTEM和rmtB基因。羊源和牛源大肠杆菌中存在β-内酰胺酶和16S rRNA甲基化酶基因介导的细菌耐药，均以blaTEM和rmtB基因为主。此外，存在不同类型耐药基因共存于同一株耐药菌的现象。

关键词: 光合有效辐射, 光合有效辐射

Abstract: The research aims at the resistant Escherichia coli carryingβ- lactamases and or 16S rRNA methylase gene and its coexistence conditions in cattle and sheep farms in Xinjiang.β-lactamase and 16S rRNA methylase genes by PCR inβ-lactam-resitance Escherichia coli were isolated from sheep farm (168 isolates) and cattle farm (32 isolates). The products of PCR were analyzed by sequencing. The results showed that the blaTEM (42.26%, 71/168) and blaCTX-M (2.98%, 5/168) genes were mainlyβ-lactamase genes from sheep; blaTEM（15.63%，5/32）genes were mainlyβ-lactamase genes from cattle. blaCTX- M was not detected, and other resistance genotypes were not seized in the two farms. ArmA genes were not detected. The detected 16S rRNA methylase genes were rmtB gene from sheep and cattle isolates, its detection rate was 4.17% (7/168) and 50.00% (16/32). Coexistence gene: three (1.79%, 3/168) isolates from cattle were found to possess the blaTEM and blaCTX-M genes in the sheep farm. seven (4.17%, 7/168) isolates from sheep were found to possess the blaTEM and rmtB genes, and two (6.25%, 2/32) isolates were found to possess the blaTEM and rmtB genes in the cattle farm. The other detectedβ- lactamase and 16S rRNA methylase genes were not found in these farms. Escherichia coli isolates from cattle and sheep farms hadβ- lactamase and 16S rRNA methylase genes mediated bacterial resistance, and were dominated by blaTEM and rmtB genes in Xinjiang. In addition, different types of drug-resistant genes coexist in the same drug-resistance bacterial strain.

底丽娜,南海辰,夏利宁,王谨. 新疆牛羊源耐药大肠杆菌β-内酰胺酶及16S rRNA甲基化酶基因检测[J]. 中国农学通报, 2014, 30(35): 87-92.

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新疆牛羊源耐药大肠杆菌β-内酰胺酶及16S rRNA甲基化酶基因检测

Detection ofβ-Lactamase and 16S rRNA Methylase Gene in Resistant Escherichia coli Isolates from Cattle and Sheep in Xinjiang

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