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中国农学通报 ›› 2014, Vol. 30 ›› Issue (34): 182-186.doi: 10.11924/j.issn.1000-6850.2014-1420

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

降解菌LW3对氯嘧磺隆污染土壤的生物修复研究

马吉平,陈庆隆,陈柳萌,王洪秀,姚 健,丁 晨   

  1. (江西省农业科学院农业应用微生物研究所,南昌 330200)
  • 收稿日期:2014-05-16 修回日期:2014-12-02 接受日期:2014-06-17 出版日期:2015-03-10 发布日期:2015-03-10
  • 通讯作者: 马吉平
  • 基金资助:
    公益性行业科研专项项目“核技术在高效、低碳农业中的应用”(201103007);公益性行业科研专项项目“主要热带作物田间废弃物综合利用技术研究与示范”(201203072);江西省自然科学基金项目“芽孢杆菌BTC-3降解丁草胺分子生物学机理的研究”(20114BAB214018)。

Bioremediation of Chlorimuron-ethyl Contaminated Soil by Strain LW3

Ma Jiping, Chen Qinglong, Chen Liumeng, Wang Hongxiu, Yao Jian, Ding Chen   

  1. (Institute of Applied Agricultural Microorganism, Jiangxi Academy of Agricultural Science, Nanchang 330200)
  • Received:2014-05-16 Revised:2014-12-02 Accepted:2014-06-17 Online:2015-03-10 Published:2015-03-10

摘要: 为了探讨添加外源接种细菌对土壤氯嘧磺隆污染修复的影响,利用从长期污染土壤分离到的氯嘧磺隆降解菌LW3,在实验室模拟情况下,研究其对氯嘧磺隆污染土壤的修复情况及其影响因素。结果表明,降解菌接种量越高,氯嘧磺隆降解效果越好,但在接种量到达1.0×106 CFU/g干土后,再增加接种量对降解率的增加效果就不太明显。土壤降解的最适温度为30℃,最适初始pH 7。在土壤含水量低于40%时,菌株对土壤氯嘧磺隆的降解率随着土壤的含水量的升高而增加;而在淹水情况下时,氯嘧磺隆的降解受到严重抑制。

关键词: 孵化指标, 孵化指标

Abstract: To investigate the effects of exogenous bacterial on soil chlorimuron-ethyl pollution remediation, a chlorimuron-ethyl degrading strain LW3 isolated from long-term contaminated soils was used to study the influencing factors of chlorimuron-ethyl reparation in soil microcosms. The results indicated that the rate of degradation was in accordance with the inoculation amount. But when the inoculation amount reached 1.0×106 CFU/g dry soil, then increase the amount of inoculum had no obvious effect on increasing the degradation rate. The optimum degradation temperature was 30℃, the optimum initial pH 7. When soil moisture content was lower than 40%, the degradation rate increased with increasing soil water content. In flooded conditions, the degradation of chlorimuron-ethyl was inhibited seriously.