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中国农学通报 ›› 2015, Vol. 31 ›› Issue (1): 43-46.doi: 10.11924/j.issn.1000-6850.2014-1668

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

杨树多基因遗传转化体系的建立及优化

朱永兴   

  1. 银川市黄河东路590号宁夏农林科学院生物中心
  • 收稿日期:2014-06-12 修回日期:2014-11-04 接受日期:2014-11-24 出版日期:2015-03-18 发布日期:2015-03-18
  • 通讯作者: 朱永兴
  • 基金资助:
    项目“作物应答高盐、低温胁迫的分子调控机理”(G2006CB100106)

Establishment and Optimization of Multi-gene Transformation system of Populus

  • Received:2014-06-12 Revised:2014-11-04 Accepted:2014-11-24 Online:2015-03-18 Published:2015-03-18

摘要: 以宁杨1号为转化受体材料,建立了杨树多基因遗传转化体系,并对SOS1-SOS2-SOS3进行了遗传转化。研究结果表明:MS 0.5 mg/L 6-BA 0.2mg/L NAA为最适不定芽分化培养基;并确定了最佳的农杆菌侵染浓度OD600=0.4,最适除草剂筛选浓度为0.8mg/L PPT,建立了最佳的宁杨1号多基因遗传转化体系,获得14株PCR阳性株系。

关键词: 分析研究, 分析研究

Abstract: In order to establish and optimize the multi-gene transformation system of Populus. ‘Ningyang No.1’ was used as acceptor material, and multi-gene transformation system was established. SOS1, SOS2 and SOS3 genes related to salt resistance were co-transformed. The results showed that the most suitable medium of adventitious bud was MS 6-BA 0.5 mg/L NAA 0.2 mg/L, the most suitable screening concentration of herbicide for the leaf piece was 0.8 mg/L, and the most suitable Agrobacterium suspension concentration was OD600=0.4. The 14 lines of PCR positive were obtained using this system.