电阻抗图谱法与电导法测定枣树枝条抗寒性的相关性

doi:10.11924/j.issn.1000-6850.2014-1905

中国农学通报 ›› 2015, Vol. 31 ›› Issue (4): 127-134.doi: 10.11924/j.issn.1000-6850.2014-1905

所属专题：园艺

电阻抗图谱法与电导法测定枣树枝条抗寒性的相关性

张振东¹,弓瑞娟²,王哲¹,高姣¹,李颖岳¹,张钢²,庞晓明¹

(¹北京林业大学林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室/生物科学与技术学院,北京 100083；²河北农业大学园艺学院,河北保定 071001）

收稿日期:2014-07-09 修回日期:2014-09-26 接受日期:2014-11-03 出版日期:2015-03-20 发布日期:2015-03-20
通讯作者: 庞晓明
基金资助:
国家自然科学基金“基于RAD标记的枣树高密度遗传图谱构建及抗寒性QTL结构作图”(31372019)；“十二五”国家科技支撑计划“鲜食枣与干制枣高效生产关键技术研究与示范”(2013BAD14B0302)。

Frost Hardiness of Chinese Jujube Shoots Assessed by Electrical Impedance Spectroscopy and Electrolyte Leakage Methods

Zhang Zhendong¹, Gong Ruijuan², Wang Zhe¹, Gao Jiao¹, Li Yingyue¹, Zhang Gang², Pang Xiaoming¹

(¹National Engineering Laboratory for Tree Breeding/ Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education/ College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083; ²College of Horticulture, Agricultural University of Hebei, Baoding Hebei 071001)

Received:2014-07-09 Revised:2014-09-26 Accepted:2014-11-03 Online:2015-03-20 Published:2015-03-20

摘要/Abstract

摘要： 选育抗寒性强的品种是避免枣树受到低温气候伤害的最有效方法，因而，探寻测定枣树抗寒性的合适方法非常重要。以7个品种枣树1年生枝条为材料，利用电导率(EL)法和电阻抗图谱(EIS)法测定其抗寒性。研究了低温胁迫下枣树枝条EIS参数（单分布电路元素电阻率r、r₁；胞外电阻率r_e；胞内电阻率r_i；弛豫时间τ；弛豫时间分布系数ψ）的变化，并对比分析了2种方法测定枣树抗寒性结果的相关性，以期确定快速测定枣树抗寒性的最佳参数。结果表明：在低温胁迫下，随着处理温度的降低，枣树枝条的各EIS参数均减小。通过EIS参数胞外电阻率r_e和弛豫时间τ拟合估算的抗寒性与EL法测定的抗寒性呈显著正相关，相关系数分别为0.840和0.879。结果表明冷冻处理后EIS参数r_e和τ可以作为测定枣树抗寒性的参数。采用EIS法测定抗寒性省时、不需要温育、不破坏植株，具有更大的优越性。

关键词: Lonsdalea quercina, Lonsdalea quercina

Abstract: Low temperature mainly limits the growth and productivity of Chinese jujube in winter and spring. The most effective way to avoid these damages in areas subjected to these climatic conditions is to select the least susceptible varieties, so the choice of the right method to determine cold hardiness is extremely important. The one-year-old shoots of seven Chinese jujube cultivars were used to measure the frost hardiness by means of electrical impedance spectroscopy (EIS) and conventional electrolyte leakage (EL) methods after exposed to controlled freezing tests. The changes of EIS parameters (specific resistances r and r₁ in Single-DCE model, specific extracellular resistance r_e, specific intracellular resistance r_i, relaxation time τ and distribution coefficient of relaxation time ψ) and the correlations between the frost hardiness results of two methods were compared in order to find the optimum EIS parameters for measuring the frost hardiness of Chinese jujube. The results showed that after controlled freezing tests, the EIS parameters decreased with the reduced temperature. The frost hardiness estimated by EIS parameters r_e and τ correlated significantly with the frost hardiness assessed by EL method (R=0.840 and 0.879, respectively). The results suggested that the parameters r_e and τ could be applied for measuring frost hardiness of the samples of Chinese jujube after exposed to controlled freezing tests. The EIS method was found to be more superior than the EL owing to its time saving, non-destructive and no need of incubation.

张振东,弓瑞娟,王哲,高姣,李颖岳,张钢,庞晓明. 电阻抗图谱法与电导法测定枣树枝条抗寒性的相关性[J]. 中国农学通报, 2015, 31(4): 127-134.

Zhang Zhendong,Gong Ruijuan,Wang Zhe,Gao Jiao,Li Yingyue,Zhang Gang and Pang Xiaoming. Frost Hardiness of Chinese Jujube Shoots Assessed by Electrical Impedance Spectroscopy and Electrolyte Leakage Methods[J]. Chinese Agricultural Science Bulletin, 2015, 31(4): 127-134.

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电阻抗图谱法与电导法测定枣树枝条抗寒性的相关性

Frost Hardiness of Chinese Jujube Shoots Assessed by Electrical Impedance Spectroscopy and Electrolyte Leakage Methods

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