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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (36): 155-160.doi: 10.11924/j.issn.1000-6850.2014-1911

• 生物技术科学 • 上一篇    下一篇

巨大芽孢杆菌C2产芽孢培养条件的优化

王继雯1,2,刘莹莹1,2,李冠杰1,2,刘 莉1,2,甄 静1,2,巩 涛1,2,杨文玲1,2,岳丹丹1,2,慕 琦2,陈国参1,2   

  1. (1河南省微生物工程重点实验室,郑州 450008；2河南科学院生物研究所有限责任公司,郑州 450008）
  • 收稿日期:2014-07-10 修回日期:2014-12-19 接受日期:2014-08-18 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 王继雯
  • 基金资助:
    2013年河南省基础前沿项目“巴西固氮螺菌定殖及其促生长机理研究”(132300413219)；2012年河南省重大科技专项“新型复合微生物肥料关键技术研究及产业化”(121100110100)；2014年河南省科学院预研项目“固氮芽孢杆菌的筛选及其竞争适应能力研究”。

Optimization of Spore Culture Conditions for Bacillus megaterium C2

Wang Jiwen1,2, Liu Yingying1,2, Li Guanjie1,2, Liu Li1,2, Zhen Jing1,2, Gong Tao1,2,Yang Wenling1,2, Yue Dandan1,2, Mu Qi2, Chen Guocan1,2   

  1. (1Key Laboratory of Microbial Engineering of Henan Province, Zhengzhou 450008;2Institute of Biology Henan Academy of Science, Zhengzhou 450008)
  • Received:2014-07-10 Revised:2014-12-19 Accepted:2014-08-18 Online:2015-03-20 Published:2015-03-20

摘要：

为了提高巨大芽孢杆菌C2产芽孢的形成率，采用单因素和正交试验，通过摇瓶发酵培养，对影响巨大芽孢杆菌C2芽孢形成的发酵培养基成分和培养条件进行了优化。结果表明：巨大芽孢杆菌C2最优的产芽孢培养基配方为麸皮1.5%、豆粕1.5%、NaCl 0.5%、CaCO3 0.02%、MnSO4·H2O 0.02%、MgSO4·7H2O 0.02%；最适宜培养条件是：培养基初始pH 7.0，接种量0.5%，培养温度37℃，培养时间48 h。在此优化条件下，巨大芽孢杆菌C2芽孢形成率可达到100%，为下一步该茵株在复合生物肥料中的应用打下基础。

关键词: 精油成分, 精油成分

Abstract: The purpose of this study is to improve the forming rate and the quantity of Bacillus megaterium C2 spores. The medial components and cultural conditions of Bacillus megaterium C2 were optimized by single factor and orthogonal tests in shake-flask fermentation. The results showed that the optimal medial components were composed of 1.5% bran, 1.5% soybean meal powder, NaCl 0.5%, 0.02% CaCO3, 0.02% MnSO4·H2O and 0.02% MgS04·7H2O. The optimal culture conditions were at temperature 37℃ with initial pH 7.0 and incubation time 48 h. Under these optimum conditions, ormation rate of Bacillus megaterium C2 achieves 100%. The foundation for the application of Bacillus megaterium as a kind of microbial fertilizer was laid.