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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (1): 150-155.doi: 10.11924/j.issn.1000-6850.2014-2078

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

抑制杨树烂皮病高效菌株的筛选及分类地位验证

李冲伟1,2,宋瑞清1   

  1. (1东北林业大学林学院,哈尔滨 150040；2黑龙江大学生命科学学院微生物学高校重点实验室,哈尔滨 150080）
  • 收稿日期:2014-07-30 修回日期:2015-01-08 接受日期:2014-11-17 出版日期:2015-03-18 发布日期:2015-03-18
  • 通讯作者: 宋瑞清
  • 基金资助:

    基金项目：国家林业局948引进项目“木霉菌株的引进”(2009-4-29)；哈尔滨市科技创新人才研究专项基金“电导法实现酒精发酵过程的在线监测”(RC2012QN002095)。

The Screening and Physiology Characteristic of
Efficient Antifungal Strain on Cytospora chrysosperma

Li Chongwei1,2, Song Ruiqing1   

  1. (1College of Forestry, Northeast Forestry University, Harbin 150040;2Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080)
  • Received:2014-07-30 Revised:2015-01-08 Accepted:2014-11-17 Online:2015-03-18 Published:2015-03-18

摘要：

为了寻找对杨树烂皮病病原菌具有高效抑制作用的木霉菌株，采用对峙培养法和生长速率法对国内外29个木霉菌株进行了筛选，并对筛选出的高效菌株的生理生态特性和分类地位进行了研究。结果表明：菌株T-33发酵液正丁醇提取物对病原菌菌丝生长的抑制率最高，达到94.2%，对孢子萌发抑制率达到100%。菌株T-33生长最适碳源为葡萄糖，最佳用量为3%(g/L)；最适氮源为黄豆粉，最佳用量为0.02%(g/L)；培养基最适初始pH 6；最适培养温度25℃。通过18S rDNA测序分析，ITS序列长度是 583 bp，菌株T-33鉴定为Trichoderma viride。

关键词: 祖代种猪场, 祖代种猪场

Abstract: In order to look for the Trichderma strains which have inhibiting effects on Chtospora chrysosperma, 29 domestic and foreign strains were screened by methods of antagonistic culture and growth-inhibiting. The morphological characteristics and physiological characteristics and taxonomic status of efficient antifungal strain were studied. The results showed that the growth-inhibiting rate of the N-butanol extract extracted from the fermented liquid of strain T-33 was 94%, and inhibition rate of spore germination was 100%. The optimal C-source of strain T-33 was glucose and the optimal content was 3% (g/L). The optimal N-source of strain T-33 was soy flour and the optimal content was 0.02% (g/L). The optimal initial pH of culture medium was 6. The optimal culture temperature was 25℃. Through 18S rDNA sequencing and phylogenies analysis of T-33, the length of ITS sequence was 583 bp, strain T-33 was verified as Trichoderma viride.