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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (6): 147-152.doi: 10.11924/j.issn.1000-6850.casb14100130

• 生物技术科学 • 上一篇    下一篇

二氯喹啉酸降解菌的培养基优化

周雨靓,张 浩,赵晓峰,侯志广   

  1. (吉林农业大学资源与环境学院,长春 130118）
  • 收稿日期:2014-10-30 修回日期:2014-11-19 接受日期:2014-11-27 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 张 浩
  • 基金资助:
    吉林省科技厅项目“玉米田除草剂残留污染的微生物修复技术研究与应用”(20120742)。

Study on the Medium Optimization of Degrading Bacteria Z2

Zhou Yuliang, Zhang Hao, Zhao Xiaofeng, Hou Zhiguang   

  1. (Resource and Environment College, Jilin Agricultural University, Changchun 130118)
  • Received:2014-10-30 Revised:2014-11-19 Accepted:2014-11-27 Online:2015-03-20 Published:2015-03-20

摘要： 为了获得二氯喹啉酸降解菌在实验室条件下的最佳基础盐培养基，为该菌的大量繁殖提供理论依据，笔者从农药厂废水处理池的淤泥中富集分离筛选出一株二氯喹啉酸降解菌(Z2)，经过鉴定该菌属于粪产碱菌属。通过单因素试验测定AOD600值确定了该菌最适繁殖的碳源和氮源，正交试验得出该菌的最佳基础盐培养基。结果表明：最适碳源与氮源分别为柠檬酸钠和酵母浸粉，最适浓度分别为0.2%和0.6%；最佳基础盐培养基为：柠檬酸钠 0.2%，酵母浸粉 0.7%，NaCl 2%，CaCl2 0.006%，FeCl3 0.0003%，K2HPO4 0.05%，KH2PO4 0.07%。在优化后的基础盐培养基中Z2能够大量繁殖，为该菌在二氯喹啉酸污染的土壤中进行修复奠定了基础。

关键词: 异形切丝, 异形切丝, 烟丝结构, 整丝率, 端部落丝量, 卷烟物理指标

Abstract: The objective of this study was to obtain the optimal basal salt medium of quinclorac degradation bacteria strain Z2 in the laboratory, which provided a theoretical basis for bacteria multiplying of Z2. Through enriching separating and screening from wastewater sludge around pesticide plant, we got quinclorac degradation bacteria strain. The bacterium, designated as Z2, was identified as Alcaligenes sp. In the single-factor test and orthogonal test, we measured the AOD600 of Z2 and finally determined the optimal basal salt medium of degradation bacteria strain Z2. The results showed that optimal carbon and nitrogen sources were sodium citrate and yeast, and the optimal basal salt medium were sodium citrate 0.2%, yeast 0.7%, NaCl 2%, CaCl2 0.006%, FeCl3 0.0003%, K2HPO4 0.05%, KH2PO4 0.07%. Z2 could multiply in the optimal basal salt medium, which laid the foundation for its use in bio-remediation of contaminated soil.