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中国农学通报 ›› 2015, Vol. 31 ›› Issue (13): 72-75.doi: 10.11924/j.issn.1000-6850.casb14110036

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

叶用莴苣组织培养再生体系的建立

黄冠华   

  1. 北京农学院
  • 收稿日期:2014-11-06 修回日期:2015-04-30 接受日期:2015-02-25 出版日期:2015-06-02 发布日期:2015-06-02
  • 通讯作者: 黄冠华
  • 基金资助:
    国家自然科学基金项目“叶用莴苣高温抽薹相关蛋白的鉴定及其互作分析”(31372057);国家自然科学基金项目“叶用莴苣高温胁迫响应因子LsHsp70的功能研究”(31401883);现代农业产业技术体系建设专项“北京市叶类蔬菜创新团队”(blvt-02);北京农学院促进人才培养综合改革专项计划“叶用莴苣高温胁迫中LsHsp70家族基因的表达模式研究”(BNRC&GG201411);北京市科技新星计划项目“叶用莴苣耐热机理的研究及耐热品种的选育”(2010B020);蔬菜创新平台建设“蔬菜创新平台建设”(5075227039);北京市农业科技项目“生菜耐热新品种繁育及安全生产技术试验示范”(20140134)。

Establishment of Tissue Culture Regeneration System of leaf Lettuce

  • Received:2014-11-06 Revised:2015-04-30 Accepted:2015-02-25 Online:2015-06-02 Published:2015-06-02

摘要: 为了满足市场需求,通过组织培养技术可以使叶用莴苣不受季节气候所牵制。随时可以工厂化大量生产,以达到百姓日食之需。本研究以PS11叶用莴苣为试材,从种子开始培育无菌苗,MS培养基为基础培养基,附加不同浓度的6-BA和NAA激素配比,再经愈伤组织诱导、芽分化、生根3个步骤的离体培养,建立叶用莴苣快速高效的再生体系。研究表明:该品种最佳诱导愈伤组织分化的培养基为MS+0.2mg/L6-BA+0.4mg/LNAA,出愈率达到100%,出芽率达到71.6%。

关键词: 室内 室外 越冬 死亡率 饲料消耗量, 室内 室外 越冬 死亡率 饲料消耗量

Abstract: In order to meet market demand, tissue culture technology is needed to lift the seasonal constrains of leaf lettuce production, and industrial mass production of the vegetable can satisfy people’ s daily need. In this study PS11 of leaf lettuce was used as test material, aseptic seedling was bred from the seeds; MS culture medium was used as basic culture medium with supplemental different concentrations of 6- BA and NAA. Through the callus induction, bud differentiation and rooting, a fast and efficient regeneration system of leaf lettuce was established. The results showed that: the best medium for callus differentiation was MS+0.2 mg/L 6-BA+0.4 mg/L NAA, the callus induction rate reached 100%, and the budding rate reached 71.6%.