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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (9): 257-260.doi: 10.11924/j.issn.1000-6850.casb15010004

• 食品 营养 检测 安全 • 上一篇    下一篇

超高压液相色谱快速测定银杏黄酮苷元

肖强,陈小强,周大寨   

  1. 生物资源保护与利用湖北省重点实验室湖北民族学院,生物资源保护与利用湖北省重点实验室湖北民族学院,生物资源保护与利用湖北省重点实验室湖北民族学院
  • 收稿日期:2015-01-04 修回日期:2015-03-09 接受日期:2015-01-28 出版日期:2015-04-07 发布日期:2015-04-07
  • 通讯作者: 肖强
  • 基金资助:
    国家自然科学基金项目“一氧化氮调控植物硒代谢机制研究”(31260057)；国家自然科学基金项目“丛枝菌根真菌对大豆硒吸收的影响机制”(31360498)；生物资源保护与利用湖北省重点实验室开放基金项目“厚朴黄连人工复合群落目的活性物质定向培育研究”(PKLHB1310)；2013年湖北省战略性新兴（支柱）产业人才培养（生物工程）项目。

Analysis of flavonol glycosides in Folium Ginkgo by UPLC-UV

  • Received:2015-01-04 Revised:2015-03-09 Accepted:2015-01-28 Online:2015-04-07 Published:2015-04-07

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摘要： 为比较鄂西南不同核用银杏品种叶片中三种主要黄酮苷元含量,采用超高压液相色谱-UV方法测定了银杏叶中黄酮苷的含量,分析柱为ZORBAX 2.1×50mm 1.8 Micron C-18 柱,流动相为乙腈-0.1%(甲酸：水),采用梯度洗脱,DAD作为检测器,检测波长360nm,保留时间定性,峰面积定量。该方法在5分钟内实现了三种黄酮苷元的快速分离,线性范围为1~12μg.mL-1,相关系数为0.9999,相对标准偏差小于2.5 %。测定结果表明,在5个核用银杏品种中,不同品种间总黄酮苷元含量具有显著差异,其中以恩银2号含量最高；各品种槲皮素含量均较低,而异鼠李素含量在总苷元中占较高比例。

关键词: 竹藤, 竹藤, 语义检索模型, 检索系统, 领域术语

Abstract: In order to compare the contents of flavonol glycosides in the leaves of Ginkgo biloba L. from different species in the southwest of hubei province, the analysis of flavonol glycosides in Folium Ginkgo by UPLC-UV was studied. The separation was performed on a ZORBAX 2.1×50mm 1.8 Micron C-18 column with acetonitrile 0.1%( formic acid: water) (with gradient) as mobile phase at a gradient flow-rate of 0.3-0.35 mL.min-1 within 7 minutes, and the column temperature of 30℃, as the detector,Sthe DAD detection wavelength was 360nm,Squalitative analysis bySretention time and quantitativeSanalysis bySpeak area. The methodSseparated three kinds ofSflavonoid glycoside within 5 minutes. The calibration curve was linear in the range of 1-12μg.mL-1, with R2 being 0.9999 and RSD being less more than 2.5%. This method is easy, rapid, accurate and suitable for the quantitative analysis of flavonol glycosides. Among the different species, the maximum contents of flavonol glycosides were found in the EnG2 species. All varieties of quercetin levels were low while isorhamnetin occupy a higher proportion.