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中国农学通报 ›› 2015, Vol. 31 ›› Issue (14): 8-13.doi: 10.11924/j.issn.1000-6850.casb15010026

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

TMR中添加牛至精油抑菌作用的研究

杨 昭,刘 婷,吴建平,雷赵民,万学瑞,何 冰   

  1. (甘肃农业大学动物科学技术学院,兰州 730070)
  • 收稿日期:2015-01-06 修回日期:2015-04-30 接受日期:2015-04-01 出版日期:2015-06-02 发布日期:2015-06-02
  • 通讯作者: 吴建平
  • 基金资助:
    兰州市科技局“兰州市高产奶牛选育策略与高校养殖技术研究师范”(2011-1-110),农业部“绒毛用羊产业技术体系放牧生态岗位科学家”(CARS-40-09B)。

Bacteriostatic Action of Oregano Essential Oil Adding to TMR

Yang Zhao, Liu Ting, Wu Jianping, Lei Zhaomin, Wan Xuerui, He Bing   

  1. (College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070)
  • Received:2015-01-06 Revised:2015-04-30 Accepted:2015-04-01 Online:2015-06-02 Published:2015-06-02

摘要: 在全混合日粮(TMR)中添加抗生素可以解决其变质问题,然而动物长期使用抗生素会产生药物残留等问题。牛至精油是一种天然无化学毒害且具有强抗菌作用的植物精油,可作为饲料添加剂。为研究牛至精油对TMR中霉菌、细菌、大肠菌群的抑制作用,并分析pH和温度对牛至精油抑菌作用的影响,将牛至精油添加到荷斯坦奶牛(约650 kg)的TMR中,采用平板菌落计数法检测TMR中霉菌、细菌的菌落数;采用国标乳糖胆盐发酵法检测大肠菌群的最可能数。结果表明:牛至精油对TMR中的霉菌、细菌、大肠菌群均有抑制作用且差异显著(P<0.05),可作为饲料添加剂提高TMR的新鲜度。

关键词: 传染性法氏囊病病毒, 传染性法氏囊病病毒, A节段, B节段, 融合PCR

Abstract: Adding antibiotics to TMR can be an effective decay control method. However, long-term use of antibiotics can produce animal drug residues and other issues. Oregano essential oil is a kind of natural essential oil which has strong antibacterial effect without chemical poison, and can be used as feed additives. This experiment was designed to study the effect of oregano essential oil on inhibiting mold, bacteria and coliform in TMR. And the effects of pH and temperature on the inhibitory effect of oregano essential oil were also analyzed. Oregano essential oil was added to TMR of Holstein cows (about 650 kg). The number of mold and bacteria colonies in TMR was counted using plate colony-counting method. Most probable number of coliforms was detected by using GB bile lactose fermentation. The results showed that oregano essential oil could inhibit mold, bacteria and coliforms in TMR and the difference was significant (P<0.05), and could be used as feed additive to improve the freshness of TMR.