用SSR标记分析‘赞皇大枣’遗传变异

doi:10.11924/j.issn.1000-6850.casb15010121

中国农学通报 ›› 2015, Vol. 31 ›› Issue (16): 94-100.doi: 10.11924/j.issn.1000-6850.casb15010121

用SSR标记分析‘赞皇大枣’遗传变异

高姣¹,刘君¹,申连英²,褚新房³,徐超群¹,杜增峰⁴,李登科⁵,王哲¹,张振东¹,庞晓明¹

(¹北京林业大学林木育种国家工程实验室/生物科学与技术学院,北京 100083；²河北农业大学中国枣研究中心,河北保定 071001；³河北省赞皇县林业局,石家庄 051230；⁴河北省沧县国家枣树良种基地,河北沧州 061000；⁵山西省农业科学院果树研究所,山西太谷 030815）

收稿日期:2015-01-18 修回日期:2015-05-22 接受日期:2015-03-23 出版日期:2015-07-27 发布日期:2015-07-27
通讯作者: 庞晓明
基金资助:
科技部“十二五”国家科技支撑计划“鲜食枣和干制枣高效生产关键技术研究与示范”(2013BAD14B0302)；国家自然科学基金委员会国家自然科学基金“基于RAD标记的枣树高密度遗传图谱构建及抗寒性QTL‘结构作图’”(31372019)。

Genetic Variation Analysis of Ziziphus jujuba ‘Zanhuang Dazao’ by SSR Markers

Gao Jiao¹, Liu Jun¹, Shen Lianying², Chu Xinfang³, Xu Chaoqun¹, Du Zengfeng⁴, Li Dengke⁵, Wang Zhe¹, Zhang Zhendong¹, Pang Xiaoming¹

(¹National Engineering Laboratory for Tree Breeding/ College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083; ²Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding Hebei 071001;³Zan huang Forestry Bureau, Shijiazhuang 051230; ⁴National Improved Cultivar Station of Jujube, Cangzhou Heibei 061000; ⁵Pomology Institute, Shanxi Academy of Agricultural Science, Taigu Shanxi 030815)

Received:2015-01-18 Revised:2015-05-22 Accepted:2015-03-23 Online:2015-07-27 Published:2015-07-27

摘要/Abstract

摘要： 研究旨在从分子水平揭示‘赞皇大枣’的遗传变异，以期为‘赞皇大枣’新品种选育提供有效的理论基础。利用SSR标记对395份‘赞皇大枣’样品进行PCR扩增，扩增产物经毛细管电泳技术检测，通过分析样品的指纹图谱数据，来评价样品间的遗传关系。16对SSR标记共扩增出51个等位基因，每个位点的等位基因数从2~9不等，平均每位点3.2个。产物片段大小范围为104~304 bp。稀有等位基因为16个，多态位点百分率为75%。395份样品中共得到28种两两相异的‘赞皇大枣’指纹图谱数据，即28种不同的基因型。对28种基因型进行聚类分析的结果表明，它们的遗传相似系数在0.7500~0.9804之间，平均值为0.9047。在遗传相似系数0.90处这些基因型被划分为5个类群。结果表明，‘赞皇大枣’群体内存在较丰富的的遗传变异，还有较大的选育出新品种的潜力。

关键词: 小麦玉米地下害虫调查防控技术, 小麦玉米地下害虫调查防控技术, 小麦玉米地下害虫调查防控技术

Abstract: Information about the genetic variation of Ziziphus jujuba ‘Zanhuang Dazao’ at molecular level can provide effective basis for selective breeding of new cultivars. 395 samples of Ziziphus jujuba ‘Zanhuang Dazao’ were collected and were amplified by PCR using 16 SSR markers. The genetic relationships among them were investigated based on analysis of fingerprint data after capillary electrophoresis. Totally, 51 alleles were detected and the number of alleles amplified by per primer ranged from 2 to 9 with an average value of 3.2. The sizes of PCR products ranged from 104 bp to 304 bp. The amount of rare alleles was 16 and polymorphic loci percentage was 75%. After clustering, 28 unique genotypes which were different from each other were obtained within 395 samples, which meant 28 different genotypes were distinguished. Further cluster analysis showed that genetic similarities among these 28 genotypes ranged from 0.7500 to 0.9804 with an average of 0.9047. At the genetic similarity of 0.90, the genotypes were classified into 5 groups. The results indicated that there was a certain degree of genetic variation among Ziziphus jujuba ‘Zanhuang Dazao’ and still had potential to select new cultivars with better quality.

高姣,刘君,申连英,褚新房,徐超群,杜增峰,李登科,王哲,张振东,庞晓明. 用SSR标记分析‘赞皇大枣’遗传变异[J]. 中国农学通报, 2015, 31(16): 94-100.

Gao Jiao,Liu Jun,Shen Lianying,Chu Xinfang,Xu Chaoqun,Du Zengfeng,Li Dengke,Wang Zhe,Zhang Zhendong and Pang Xiaoming. Genetic Variation Analysis of Ziziphus jujuba ‘Zanhuang Dazao’ by SSR Markers[J]. Chinese Agricultural Science Bulletin, 2015, 31(16): 94-100.

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用SSR标记分析‘赞皇大枣’遗传变异

Genetic Variation Analysis of Ziziphus jujuba ‘Zanhuang Dazao’ by SSR Markers

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