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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (20): 35-38.doi: 10.11924/j.issn.1000-6850.casb15010228

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

X射线灭活活体日本沼虾精子的初步研究

颜跃弟1,傅洪拓1,2,梁国霞3,乔 慧2,白鸿坤3,蒋速飞2,熊贻伟2,龚永生2,金舒博2,张文宜2,吕 洋3,吴滟2   

  1. (1上海海洋大学水产与生命学院,上海 200000；2中国水产科学研究院淡水渔业研究中心/农业部淡水渔业和种质资源利用重点实验室,江苏无锡 214081；3南京农业大学无锡渔业学院,江苏无锡 214081）
  • 收稿日期:2015-01-29 修回日期:2015-06-29 接受日期:2015-04-24 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 傅洪拓
  • 基金资助:
    江苏省科技支撑计划-农业部分“优质高产抗逆青虾新品种的选育”(BE2012334)；国家“十二五”支撑“青虾新品种选育技术研究”(2012BAD2604-05)；江苏省基础研究计划（自然科学基金）青年基金“青虾精巢发育调控基因的鉴别、克隆及表达研究”(BK2012091)；江苏省三新工程工程项目“青虾新品种选育”(D2013-6)；中央级公益性科研院所基本科研业务费专项资金项目“青虾性成熟相关基因的筛选及功能研究”(2015JBFM05)。

Effect of X-ray on Inactivating Sperms by Radiating Intravital Macrobrachium nipponensis

Yan Yuedi1, Fu Hongtuo1,2, Liang Guoxia3, Qiao Hui2, Bai Hongkun3, Jiang Sufei2, Xiong Yiwei2, Gong Yongsheng2, Jin Shubo2, Zhang Wenyi2, Lv Yang3, Wu Yan2   

  1. (1College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200000;2Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Wuxi Jiangsu 214081;3Wuxi Fishery College, Nanjing Agricultural University, Wuxi Jiangsu 214081)
  • Received:2015-01-29 Revised:2015-06-29 Accepted:2015-04-24 Online:2015-07-28 Published:2015-07-28

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摘要： 为检测X射线对日本沼虾精子的灭活效果，利用X射线对雄性日本沼虾进行不同时间的照射处理，将处理后的雄虾与刚完成生殖脱壳的雌虾交配。待雌虾产卵后，计数雌虾抱卵天数，并在显微镜下观察卵的畸形情况。X射线管照射强度为100kV，5mA，源距10cm，照射时间为0、30、60、90、120min。结果发现：照射30min组的抱卵天数及卵的畸形率较0min组无变化，卵的畸形率为0，卵能正常发育至孵化出膜。随着照射时间的增加，在30~90min之间，雌虾抱卵天数逐渐减少，且所抱卵的畸形率呈上升趋势。照射120min组的抱卵天数较90min组变化不大，卵的畸形率达100%。在本试验照射时间范围(0~120min)内，灭活日本沼虾精子遗传物质的最佳照射时间为90~120min。

关键词: 农业产业化企业, 农业产业化企业, 战略联盟, 稳定性影响因素

Abstract: To investigate the effect of X-ray on sperm inactivation of Macrobrachium nipponense, male shrimps were irradiated by 100 kV-5 mA X-ray for 0, 30, 60, 90, 120 min with a distance of 10 cm. Then the male shrimps were made to mate with female soft-shelled shrimps. Broody days and aberration rate were recorded after ovulation. The results showed that there was no difference between 0 min group and 30 min group, and the aberration rate was 0. Broody days gradually decreased while aberration rate increased with increasing irradiation time from 30 to 90 min. After 90 min, broody days showed no change, and the aberration rate reached a peak of 100%. In this research, the radiation time range was 0-120 min. According to the results, the optimum irradiation time to inactivate genetic material of sperms of Macrobrachium nipponense was 90- 120 min.