土壤水分胁迫下茶树部分渗透调节物质的变化

doi:10.11924/j.issn.1000-6850.casb15030206

中国农学通报 ›› 2015, Vol. 31 ›› Issue (28): 126-131.doi: 10.11924/j.issn.1000-6850.casb15030206

所属专题：园艺

土壤水分胁迫下茶树部分渗透调节物质的变化

郭春芳^1,2,罗玲娜²,何水平²,孙云²

(¹福建教育学院,福州 350025；²福建农林大学园艺学院,福州 350002）

收稿日期:2015-03-25 修回日期:2015-05-15 接受日期:2015-05-22 出版日期:2015-10-27 发布日期:2015-10-27
通讯作者: 孙云
基金资助:
福建省自然科学基金项目“茶树水分高效利用的分子生理基础研究”(B0510025)、“茶树脯氨酸代谢关键基因δ-OAT和P5CS克隆及功能分析”(2011J01091)。

Changes of Several Osmotica in Tea Plant (Camellia sinensis) Under Soil Water Stress

Guo Chunfang^1,2, Luo Lingna², He Shuiping², Sun Yun²

(¹Fujian Institute of Education, Fuzhou 350025;²College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002)

Received:2015-03-25 Revised:2015-05-15 Accepted:2015-05-22 Online:2015-10-27 Published:2015-10-27

摘要/Abstract

摘要： 为了了解不同茶树品种对干旱环境的适应性，分析了土壤水分胁迫对茶树部分渗透调节物质含量变化的影响。以茶树‘铁观音’和‘福鼎大白茶’2年生幼苗为材料，采用盆栽试验的方法，研究了在正常供水（土壤含水量为田间最大持水量的75%）、轻度水分胁迫(55%)、中度水分胁迫(35%)和重度水分胁迫(20%)条件下，茶树部分渗透调节物质的变化情况。结果表明，正常供水条件下，‘铁观音’叶片相对含水量、脯氨酸含量低于‘福鼎大白茶’，可溶性蛋白和可溶性糖含量高于‘福鼎大白茶’；水分胁迫下‘铁观音’、‘福鼎大白茶’叶片相对含水量随胁迫程度的加大而降低，可溶性蛋白质、可溶性糖以及脯氨酸的含量随胁迫程度的加大而增多，但在重度胁迫下，‘福鼎大白茶’的可溶性蛋白质及脯氨酸含量下降。在相同的水分胁迫条件下，‘铁观音’叶片相对含水量的降幅都小于‘福鼎大白茶’，而可溶性蛋白、可溶性糖和脯氨酸含量的增幅都比‘福鼎大白茶’大。表明在土壤水分胁迫下‘铁观音’的渗透调节适应能力高于‘福鼎大白茶’。

关键词: 新生代农民工, 新生代农民工, 未来认同, 身份认同

Abstract: In order to understand the adaptability of different varieties of tea plants to drought environment, the contents of several osmotica in tea plants under soil water stress were studied. Two-year-old seedlings of tea plants (Camellia sinensis cv. Tieguanyin and C.sinensis cv. Fudingdabaicha) were cultivated in a pot experiment to investigate the changes of several osmotica under four water stress treatments: 75% (normal water supply), 55% (slight water stress), 35% (mild water stress) and 20% (severe water stress) of soil field capacity. The results showed that the relative water contents (RWC) and free proline contents of Camellia sinensis cv. Tieguanyin were lower than that of Camellia sinensis cv. Fudingdabaicha, while the soluble protein and soluble sugar content were higher than that of Camellia sinensis cv. Fudingdabaicha under normal water supply conditions. Under water stress conditions, the RWC of Camellia sinensis cv. Tieguanyin and C.sinensis cv. Fudingdabaicha decreased, and the contents of soluble protein, soluble sugar and proline increased along with the higher of the stress degree. But under the severe water stress, soluble protein and proline contents of C.sinensis cv. Fudingdabaicha decreased. In the same water stress conditions, the decreasing RWC amplitude of Camellia sinensis cv. Tieguanyin was less than that of C.sinensis cv. Fudingdabaicha, and the increasing amplitude of soluble protein, soluble sugar and proline contents were higher than that of C.sinensis cv. Fudingdabaicha. Compared with C.sinensis cv. Fudingdabaicha, C.sinensis cv. Tieguanyin had higher capacity to acclimate soil water stress.

中图分类号:

S571.1

郭春芳,罗玲娜,何水平,孙云. 土壤水分胁迫下茶树部分渗透调节物质的变化[J]. 中国农学通报, 2015, 31(28): 126-131.

Guo Chunfang,Luo Lingna,He Shuiping and Sun Yun. Changes of Several Osmotica in Tea Plant (Camellia sinensis) Under Soil Water Stress[J]. Chinese Agricultural Science Bulletin, 2015, 31(28): 126-131.

参考文献

[1] Hisao T C. Water and plant life [M]. New York:Academic Press, 1973:281-303.
[2] Hisao T C. Stress metabolism: water stress, growth, and osmotic adjustment[J]. Phil. Trans. R. soc. Lond. B,1976,273:479-500.
[3] Westgate M E, Boyer T S. Osmotic adjustment and the inhibition of leaf,root and silk growth at low water potentials in maize[J].Planta, 1985(164):540-549.
[4] Taylor C B. Proline and water deficit: ups, downs, ins and outs[J].Plant Cell,1996,8:1221-1224.
[5] 汤章城.不同抗旱品种高粱苗中脯氨酸积累的差异[J].植物生理学报,1986,12(2):154-162.
[6] 裴英杰,郑家玲,庚红,等.用于玉米品种抗旱性鉴定的生理生化指标[J].华北农学报,1992,7(1):32-35.
[7] 张立军.渗透胁迫下玉米幼苗离体叶片膜透性变化机理的研究[J].沈阳农业大学学报,1996,27(3):207-210.
[8] 宗会,刘娥娥,郭振飞.干旱、盐胁迫下LaCl₃和CPZ对稻苗脯氨酸积累的影响[J].作物学报,2001,27(2):173-177.
[9] 刘娥娥,宗会.干旱和低温胁迫对水稻幼苗脯氨酸含量积累的影响[J].亚热带植物学报,2000,8(3):235-238.
[10] Jiang M Y, Guo S C, Zhang X M. Proline accumulation in rice seedings exposed to hydroxyl radical stress in relation to antioxidation[J].Chin.Sci.Bull,1997(42):855-859.
[11] 李德全,邹琦,程炳嵩.抗旱性不同的冬小麦品种渗透调节能力的研究[J].山东农业大学学报,1991,22(4):377-383.
[12] 汪沛洪,山仑,王葆莉,等.干旱逆境下药剂处理小麦种子对灌浆期某些生理影响与产量形成的关系[J].干旱地区农业研究,1995,13(3):58-66.
[13] 上官周平,陈培元.土壤干旱对小麦叶片渗透调节作用和光合作用的影响[J].华北农学报,1989,4(4):44-49.
[14] 黎裕.植物的渗透调节与其它生理过程的关系及其在作物改良中的应用[J].植物生理学通讯,1994,30(5):377-385.
[15] 王守生.茶树游离脯氨酸含量及水分胁迫对其影响[J].茶叶,1995,1:22-25.
[16] Manivel L. Shoot water potential in teaⅠ: Standardization of sampling[J].Two and a bud,1983,30(2):77-81.
[17] Handique A C. Shoot water potential in teaⅡ: Screening Tocklai cultivars for drought tolerance [J]. Two and a bud,1986,33(2):39-42.
[18] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:11-160.
[19] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical biochemistry, 1976,72:248-254.
[20] 汤章城.现代植物生理学实验指南[M].北京:科学出版社,2003:127-217.
[21] 张殿忠,汪沛洪,赵会贤.测定小麦叶片游离脯氨酸含量的方法[J].植物生理学通讯,1990(4):62-65.
[22] Lu D B, Yang J P, Li L Z. Correlative study on the physiological reaction and yield performance of wheat varieties stressed by water deficiency[J].Acta Agriculturae Universitatis Henanensis,1994,3:230-234.
[23] 李合生.现代植物生理学[M].北京:高等教育出版社,2002:130-135.
[24] 张明生,谢波,谈锋,等.甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究[J].中国农业科学,2003,36(1):13-16.
[25] 李妮亚,高俊风,汪沛洪.小麦幼苗水分胁迫诱导的蛋白质的特征[J].植物生理学报,1998,24(1):65-71.
[26] Singh N K. Drought risistence in plant[J].Plant Physiol,1987(79):126-137.
[27] Zhang X Z. Physiological Research Method[M].Beijing: Agricultural Publishing Press,1992:91-95.
[28] 张美云,钱吉,郑师章.渗透胁迫下野生大豆游离脯氨酸和可溶性糖的变化[J].复旦学报:自然科学版,2001,40(5):558- 561.
[29] Singh T N, Aspinall D, Paleg L G. Proline accumulation and varical adaptability to drought in barley: a potential metabolic measure of drought resistance[J].Nature New Bio1.,1972,236:188- 190.
[30] 汤章城.逆境条件下植物脯氨酸的累积及其可能的意义[J].植物生理学通讯,1984,1:15-21.
[31] 蒋明义,郭绍川.氧化胁迫下稻苗体内积累的脯氨酸的抗氧化作用[J].植物生理学报,1997,23(4):347-352.
[32] 伍炳华,韩文炎,姚国坤.茶树对土壤干旱的生理反应[J].中国茶叶,1991,6:2-3.
[33] 王守生.茶树游离脯氨酸含量及水分胁迫对其影响[J].茶叶,1995,1:22-25.
[34] 吴伯千,潘根生.茶树对水分胁迫的生理生化反应[J].浙江农业大学学报,1995,21(5):451-456.
[35] 李华钧.等渗透胁迫对茶树幼苗叶片脯氨酸累积和水分含量的影响[J].西南农业大学学报,1993,8:119-122.
[36] Handique A C. Shoot water potential in teaⅡ: Screening Tocklai cultivars for drought tolerance [J]. Two and a bud,1986,33(2):39-42.
[37] 李金昌.旱热季节不同唢水培对秋茶产量和品质的影响[J].中国茶叶,1987(5):2-4.

土壤水分胁迫下茶树部分渗透调节物质的变化

Changes of Several Osmotica in Tea Plant (Camellia sinensis) Under Soil Water Stress

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	王冬,刘养卉. 新生代农民工创业行为影响因素分析——基于兰州市的调查[J]. 中国农学通报, 2015, 31(5): 278-283.
[2]	赵娜刘霞. 新生代农民工的未来认同及其影响因素研究——基于青岛的问卷[J]. 中国农学通报, 2014, 30(23): 112-118.
[3]	夏燚. 新生代农民工的社会保障权益问题研究——基于苏州328名新生代农民工的调查[J]. 中国农学通报, 2014, 30(17): 151-155.
[4]	王树娟. 鲁西地区新生代农民工教育培训需求分析[J]. 中国农学通报, 2012, 28(20): 221-225.