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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (16): 123-131.doi: 10.11924/j.issn.1000-6850.casb15080038

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

辣椒疫霉不同发育阶段对氟吗啉的敏感性研究

何烈干1,葛艳丽2,马辉刚1,陈学军3,方 荣3   

  1. (1江西省农业科学院植物保护研究所,南昌 330200；2江西生物科技职业学院,南昌 330200；3江西省农业科学院蔬菜花卉研究所,南昌 330200）
  • 收稿日期:2015-08-05 修回日期:2016-02-27 接受日期:2016-03-24 出版日期:2016-06-17 发布日期:2016-06-17
  • 通讯作者: 马辉刚
  • 基金资助:
    江西省现代农业科研协同创新专项经费资助“蔬菜新品种引育及优质安全高效栽培关键技术研究与示范”(JXXTCX2015005-004)；江西省科技支撑重点项目“辣椒疫病菌对杀菌剂的抗药性及治理对策研究”(2010BNA04900)；国家公益性行业（农业）科研专项“蔬菜卵菌病害综合防控技术研究与示范”(201003004)。

Sensitivity of Different Morpha of Phytophthora capsici to Flumorph

He Liegan1, Ge Yanli2, Ma Huigang1, Chen Xuejun3, Fang Rong3   

  1. (1Plant Protection Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200; 2Jiangxi Biotech Vocational College, Nanchang 330200; 3Vegetable and Flower Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200)
  • Received:2015-08-05 Revised:2016-02-27 Accepted:2016-03-24 Online:2016-06-17 Published:2016-06-17

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摘要： 从江西省各地采集分离获得108个辣椒疫霉菌株，通过菌丝生长法等测定了辣椒疫霉菌的不同生长阶段对氟吗啉的敏感性。研究结果表明，氟吗啉对辣椒疫霉菌的不同生长发育阶段的抑制作用明显，辣椒疫霉菌丝生长对氟吗啉的敏感性较高，EC50值范围为0.07852~0.38622 μg/mL，平均EC50值为0.18159 μg/mL；游动孢子囊形成对氟吗啉的敏感性更高，EC50值范围为0.00251~0.03943 μg/mL，平均EC50值为0.01588 μg/mL；游动孢子萌发对氟吗啉的敏感性最高，EC50值范围为0.00156~0.00965 μg/mL，平均EC50值为0.00515 μg/mL。正态分析表明，辣椒疫霉菌108个菌株的3个发育阶段对氟吗啉的敏感性符合正态分布，江西省内目前还不存在耐药性的辣椒疫霉群体。

关键词: 杜鹃花属, 杜鹃花属, 条形码技术, ITS基因序列, 系统进化树

Abstract: 108 single-sporangia isolates of Phytophthora capsici, which can cause capsicum blight, were collected from several areas in Jiangxi Province and purified, the bioactivity of flumorph against different stages of Phytophthora capsici was compared in vitro. The results showed that flumorph had significant inhibitory effect on the 3 different growth stages of the strain, mycelial growth of pathogen was affected by flumorph, with a mean EC50 as 0.18159 μg/mL, ranging from 0.07852 μg/mL to 0.38622 μg/mL. The producing of sporangia was more sensitive to flumorph, with a mean EC50 as 0.01588 μg/mL, ranging from 0.00251 μg/mL to 0.03943 μg/mL. The germination of zoospores of Phytophthora capsici was most sensitive to flumorph, and EC50 distributed from 0.00156 to 0.00965 μg/mL with a mean value of 0.00515 μg/mL. Normal distribution analysis showed that the sensitivity of these isolates to flumorph was fitted well with a normal distribution function, and there was no strain of capsicum blight pathogen that had resistance to the fungicide.