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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (22): 32-36.doi: 10.11924/j.issn.1000-6850.casb15110136

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

珍稀濒危植物沙冬青的组织培养

王方琳1,2,柴成武1,尉秋实1,魏小红2,马俊梅1,李爱德1,王昱淇1,   

  1. (1甘肃省治沙研究所/甘肃省荒漠化与风沙灾害防治重点实验室,甘肃武威 733000；2甘肃农业大学生命科学技术学院,兰州 730070）
  • 收稿日期:2015-11-26 修回日期:2016-08-01 接受日期:2016-05-31 出版日期:2016-08-09 发布日期:2016-08-09
  • 通讯作者: 王方琳
  • 基金资助:
    国家自然科学基金项目“固定流沙先锋树种沙生柽柳无性繁殖生根机制研究”(31460069) ；国家自然科学基金项目“外源一氧化氮调控紫花苜蓿抗寒性的信号转导机制研究”(31560663)；国家自然科学基金项目“风沙流对民勤荒漠灌木幼苗形态特征的影响及其生理生化响应机制” (31460224)；国家自然科学基金项目“石羊河下游绿洲荒漠过渡带微区集水过程及其植被效应研究”(41161006)；国家自然科学基金项目“石羊河中下游退耕地土壤系统演变规律及其驱动机制研究”(41161049)。

Tissue Culture of Endangered Psammophyte Ammopiptanthus mongolicus

张莹花1,张锦春1,丁春发2,赵 颖2Wang Fanglin1,2, Chai Chengwu1, Yu Qiushi1, Wei Xiaohong2, Ma Junmei1, Li Aide1, Wang Yuqi1, Zhang Yinghua1, Zhang Jinchun1, Ding Chunfa2, Zhao Ying2   

  1. (1The State Key Laboratory of Desertification Combating Prevention and Sandstorm Disaster of Gansu Province/Gansu Desert Control Research Institute, Wuwei Gansu 733000;2College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070)
  • Received:2015-11-26 Revised:2016-08-01 Accepted:2016-05-31 Online:2016-08-09 Published:2016-08-09

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摘要： 试验以珍稀濒危植物沙冬青无菌苗的子叶、茎段为外植体材料，研究不同激素配比的培养基对不同外植体愈伤组织培养、丛生芽增殖及生根培养的影响，并筛选适宜各阶段培养的最佳培养基配方。结果表明，子叶是诱导沙冬青愈伤组织的最好外植体，在6-BA 0.5 mg/L、2,4-D 0.5 mg/L时，其愈伤组织诱导率达到最大值81.8%；在6-BA 2.0 mg/L、NAA 0.5 mg/L时丛生芽增殖率达到最大值90.2%，且丛生芽数量多、茎干健壮、生长旺盛，说明较高浓度的细胞分裂6-BA对丛生芽增殖具有明显的促进作用；在以1/2MS为基本培养基，加入NAA 1.0 mg/L时，生根率达到最大值70.4%，且生根时间最早，主根明显、粗壮且根毛数量多；适当调节培养基中无机盐的浓度提高生根率是将来沙生植物组织培养中研究的重点和难点。

关键词: 柑橘病原菌, 柑橘病原菌, 柑橘保鲜, 中草药提取物, 川芎

Abstract: The paper aims to test effects of medium with different hormone combinations on explants callus culture, buds proliferation and rooting culture of rare and endangered plants Ammopiptanthus with aseptic cotyledons and stem segments as explants materials, and screen the optimum medium formula for each culture stage. The results showed that cotyledon was the best explants to induct callus of Ammopiptanthus. The callus induction rate reached its maximum 81.8% at 6-BA 0.5 mg/L, 2,4-D 0.5 mg/L. The clustered buds proliferation rate reached the maximum of 90.2% at 6-BA 2.0 mg/L, NAA 0.5 mg/L. The phenomenon of multiple clustered buds, robust stem and vigorous growth was observed, indicating that higher concentrations of cell division 6-BA could significantly promote cluster bud proliferation. The rooting rate reached the maximum of 70.4% in 1/2MS basic medium added with NAA 1.0 mg/L, the rooting time was the earliest, with obvious main root, which was stout and hairy. The key point in psammophytes tissue culture research will be adjusting inorganic salt concentration in the medium properly to improve the rooting rate.