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中国农学通报 ›› 2017, Vol. 33 ›› Issue (7): 104-111.doi: 10.11924/j.issn.1000-6850.casb16050024

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

不同颜色蝴蝶兰花瓣表皮细胞形态差异比较

肖文芳,李 佐,陈和明,吕复兵   

  1. 广东省农业科学院环境园艺研究所,广东省农业科学院环境园艺研究所,广东省农业科学院环境园艺研究所,广东省农业科学院环境园艺研究所
  • 收稿日期:2016-05-05 修回日期:2017-01-17 接受日期:2016-07-29 出版日期:2017-03-16 发布日期:2017-03-16
  • 通讯作者: 吕复兵
  • 基金资助:
    广东省科技计划项目“蝴蝶兰在西藏林芝地区反季节栽培技术研究”(2014A020209065);广东省省级科技计划项目“中小花型蝴蝶兰新品 种选育”(2014A020208063);广东省省级科技计划项目“兰花种质创新及优异种质规模化高效扩繁技术研发与应用”(2015B020231005);广东省省级 科技计划项目“西藏林芝地区野生兰花资源调查、收集与利用研究”(2013B020315001);广东省省级科技计划项目“蝴蝶兰优异种质产业化利用的关 键技术研发与示范平台建设”(2016B070701014)。

Comparing the Petal Microstructure of Different Color Phalaenopsis

  • Received:2016-05-05 Revised:2017-01-17 Accepted:2016-07-29 Online:2017-03-16 Published:2017-03-16

摘要: 为探究不同颜色蝴蝶兰花瓣表皮细胞形态的差异性,采用扫描电子显微镜分别对8个不同颜色的纯色蝴蝶兰品种的花瓣中心部位(2个白色蝴蝶兰品种,3个黄绿色蝴蝶兰品种和3个紫红色蝴蝶兰品种)和4个有斑的蝴蝶兰品种(2个白底红斑的蝴蝶兰品种和2个黄底红斑的蝴蝶兰品种)的底色部位、斑色部位及底色与斑色相交部位分别进行观察对比,同时采用鲜切片浸泡在0.25% PEG溶液中观察有斑样品斑纹交界处的纵切面表皮细胞形状变化。结果发现,除2个白色品种的表皮细胞为乳突状外,其他品种的表皮细胞均为穹顶状。另外发现部分样品花瓣表皮存在少量气孔。结果表明,相同颜色不同品种的蝴蝶兰花瓣表皮细胞形状可能不同,但同一片花瓣不同颜色区域的表皮细胞结构则是相同的,说明花瓣颜色并不是影响蝴蝶兰花瓣表皮细胞形状的决定因素。以上不同颜色蝴蝶兰花瓣显微结构的观察比较,将对蝴蝶兰花色研究提供一定的参考。

关键词: 苦瓜育苗, 苦瓜育苗, 营养钵育苗, 穴盘育苗, 营养基质块育苗, 幼苗质量

Abstract: This study designed to explore the relationship between flower color and petal epidermal cells shape in Phalaenopsis. Scanning electron microscopy (SEM) was used to explore the petal microstructure differences of Phalaenopsis. According to the Royal Horticultural Society Color Chart (RHSCC), flower color of 8 evenly colored flowers were grouped into four classes: white, green-yellow, purple and purple-violet. In this research, evenly colored flowers, including two white samples, three green-yellow samples, two purple-violet samples and one purple sample, four purple-blotched flowers with white ground color and yellow ground color were studied using SEM, respectively. Three different parts, including yellow or white ground color parts, purple blotch parts and the junction of the ground color and the over color, in the blotched flowers were observed. In addition, transverse section from the middle of the petal of the junction of the ground color and the over color in each spotted flowers were taken using a sharp razor blade, mounted in 0.25% PEG solution which had an osmotic potential close to that of petal cells, and examined using a Zeiss microscope. [Result] The two white flowers had papillate epidermal cells, all the other 10 flowers had dome-shaped epidermal cells. In addition, there was a small amount of pores in the petal epidermis of some samples. The epidermal cell shape of the same color from the different varieties of Phalaenopsis petals may be different (papillate or dome), but the cell shape in different color regions of the same petal is the same. These finding prove that flower color, no matter ground color or over color, is not the determinant effect of the petal epidermal cell shape in Phalaenopsis. The above comparisons study between different color Phalaenopsis will provide some reference for the future research about color in Phalaenopsis.

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