茶多酚和槲皮素对炎性脂肪细胞信号通路和炎性因子的影响

doi:10.11924/j.issn.1000-6850.casb16110097

中国农学通报 ›› 2018, Vol. 34 ›› Issue (5): 25-30.doi: 10.11924/j.issn.1000-6850.casb16110097

所属专题：生物技术；园艺

茶多酚和槲皮素对炎性脂肪细胞信号通路和炎性因子的影响

檀昕,程安玮

山东省农业科学院农产品研究所,山东省农业科学院农产品研究所

收稿日期:2016-11-18 修回日期:2017-01-17 接受日期:2017-02-24 出版日期:2018-02-26 发布日期:2018-02-26
通讯作者: 程安玮
基金资助:
山东省农业科学院重大科技成果培育计划“果蔬原料中天然活性物质研究及产业化”(2016CGPY09)。

Effects of Tea Polyphenols and Quercetin on Inflammatory Adipose Cell Signaling Pathways and Inflammatory Factor

Received:2016-11-18 Revised:2017-01-17 Accepted:2017-02-24 Online:2018-02-26 Published:2018-02-26

摘要/Abstract

摘要： 旨在研究茶多酚(TP)、槲皮素(Que)及两者协同作用时的抗炎作用机理。以TNF-α 诱导的炎性3T3-L1 脂肪细胞为模型，分别添加50 μg/mL 茶多酚(TP)、50 μg/mL 槲皮素(Que)及50 μg/mL 的茶多酚和槲皮素(TP+Que)共同处理，以空白组作为对照组。研究脂肪细胞中炎性因子白介素12(IL-12)、一氧化氮合酶(iNOS)的基因表达，并采用Western blot 研究其对Sirt1 和AMPK的磷酸化水平的影响。结果表明：在TNF-α 刺激条件下，茶多酚和槲皮素对炎性因子的基因表达和炎症信号通路都有着明显的抑制作用，但槲皮素的抑制作用则不如茶多酚显著。在脂肪细胞中，活性物质可通过AMPK/Sirt1 途径，调节IL-12和iNOS的基因表达发挥抗炎作用。

关键词: 石河子地区, 石河子地区, Penman-Monteith模型, 极端干湿事件

Abstract: The aim is to investigate the anti-inflammatory mechanism of tea polyphenols (TP), quercetin (Que) and their synergy. The inflammatory of 3T3- L1 adipocyte induced by TNF- α was used as model. Tea polyphenols (50 μg/mL TP), quercetin (50 μg/mL Que), tea polyphenols and quercetin (50μg/mL of Que+TP) were added to the inflammatory of 3T3-L1 adipocytes respectively, the blank group was used as the control group. The gene expressions of interleukin 12 (IL-12) and nitric oxide synthase (iNOS) in adipocyte were studied. The effects on the phosphorylation level of AMPK and Sirt1 were also studied using the western blot. The results showed that the TP and Que had significant inhibition on gene expression and inflammatory signaling pathways in the condition of TNF-α stimulation, however, the effect of Que was not as significant as that of TP. In adipocytes, the active substance regulated the expression of IL-12 and iNOS by the AMPK / Sirt1 pathway to exert anti-inflammatory effects.

檀昕,程安玮. 茶多酚和槲皮素对炎性脂肪细胞信号通路和炎性因子的影响[J]. 中国农学通报, 2018, 34(5): 25-30.

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茶多酚和槲皮素对炎性脂肪细胞信号通路和炎性因子的影响

Effects of Tea Polyphenols and Quercetin on Inflammatory Adipose Cell Signaling Pathways and Inflammatory Factor

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