新疆野扁桃自交不亲和SFB与S-RNase蛋白间相互作用的研究

doi:10.11924/j.issn.1000-6850.casb16120056

中国农学通报 ›› 2017, Vol. 33 ›› Issue (14): 33-38.doi: 10.11924/j.issn.1000-6850.casb16120056

所属专题：生物技术；园艺

新疆野扁桃自交不亲和SFB与S-RNase蛋白间相互作用的研究

曾斌,关鹏,夏江宏,刘梦雯,王波

新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心,新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心,新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心,新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心,新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心

收稿日期:2016-12-12 修回日期:2017-05-04 接受日期:2017-02-24 出版日期:2017-05-25 发布日期:2017-05-25
通讯作者: 曾斌
基金资助:
国家自然科学基金“基于S-核酸酶的新疆野生扁桃自交不亲和性泛素化特性的分子机制研究(31660557)”,“新疆野生扁桃自交不亲和性的分子机制研究”(31260465)；新疆维吾尔自治区果树学重点学科项目“新疆特色果树种质资源开发利用与优良品种选育推广” (201007)。

The Interaction Between SFB and S-RNase Protein Controlling Self-incompatibility in Xinjiang Wild Almond (Prunus tenella Batsch.)

Received:2016-12-12 Revised:2017-05-04 Accepted:2017-02-24 Online:2017-05-25 Published:2017-05-25

摘要/Abstract

摘要： 利用酵母双杂交系统研究新疆野扁桃自交不亲和性花粉SFB蛋白与花柱S-RNase蛋白间的相互作用。以野扁桃花药为实验材料，根据实验要求和基因的结构特点，利用已克隆到的SFB 和S-RNase 基因，有针对性的截短，构建酵母双杂交载体。研究结果显示，成功构建了酵母双杂交重组表达载体，PtSFB17、PtS16-RNase 和PtS17-RNase 各蛋白之间并没有发现存在相互作用，据此推测PtS16-RNase 蛋白和PtS17-RNase 蛋白不在PtSFB17 蛋白的识别谱中，协同非我识别模型在新疆野扁桃中同样存在。新疆野扁桃自交不亲和性PtSFB17、PtS16-RNase和PtS17-RNase各蛋白间并无相互作用。

关键词: 花黄斑蜂, 花黄斑蜂, COⅠ基因, 基因条码

Abstract: The yeast two-hybrid system was used to study the interaction between pollen SFB and column SRNase protein of self-incompatibility in Xinjiang wild almond. Taking anther from Xinjiang wild almond as material, according to the experimental requirements and structure characteristics of gene, the cloned genes SFB andS-RNase were truncated and then used to construct the yeast two hybrid recombinant expression vectors. The results showed that the yeast two hybrid recombinant expression vectors were constructed successfully. There was no interaction between PtSFB17 protein and PtS16-RNase protein or PtS17-RNase protein. Based on the above, it was presumed that PtS16-RNase protein and PtS17-RNase protein were not in the identification spectrum of PtSFB17 protein. Collaborative non-self recognition system was also in Xinjiang wild almond. There was no interaction between PtSFB17 protein and PtS16-RNase protein or PtS17-RNase protein of self incompatibility in Xinjiang wild almond.

中图分类号:

S662.9

曾斌,关鹏,夏江宏,刘梦雯,王波. 新疆野扁桃自交不亲和SFB与S-RNase蛋白间相互作用的研究[J]. 中国农学通报, 2017, 33(14): 33-38.

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新疆野扁桃自交不亲和SFB与S-RNase蛋白间相互作用的研究

The Interaction Between SFB and S-RNase Protein Controlling Self-incompatibility in Xinjiang Wild Almond (Prunus tenella Batsch.)

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