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中国农学通报 ›› 2017, Vol. 33 ›› Issue (22): 138-144.doi: 10.11924/j.issn.1000-6850.casb17040124

所属专题: 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

三种豆科牧草愈伤组织继代培养中抗褐化的研究

陈爱萍,冯维卫,刘允康   

  1. 新疆农业大学 新疆草地资源与生态实验室,新疆农业大学 新疆草地资源与生态实验室,新疆农业大学 新疆草地资源与生态实验室
  • 收稿日期:2017-04-19 修回日期:2017-05-12 接受日期:2017-05-25 出版日期:2017-08-21 发布日期:2017-08-21
  • 通讯作者: 陈爱萍
  • 基金资助:
    草业科学国家重点学科项目“提高苜蓿原生质体细胞分裂频率的研究”(XJCYQ-2012-02)。

Anti-browning Agents Affecting Callus Subculture of Three Legume Forage Species

  • Received:2017-04-19 Revised:2017-05-12 Accepted:2017-05-25 Online:2017-08-21 Published:2017-08-21

摘要: 为解决3 种豆科牧草愈伤组织继代培养中的褐化问题,以‘新牧4 号’紫花苜蓿、普通红豆草和‘海法’三叶草愈伤组织为材料,探讨不同防褐化措施在3 种豆科牧草愈伤继代培养过程中抗褐化效果的影响。结果表明,在苜蓿和红豆草愈伤组织的继代培养中,对愈伤组织褐化抑制效果较好的是AgNO3,浓度为0.02 g/L 时,褐化率分别为13%、15%,愈伤组织褐化程度强弱依次是PVP>柠檬酸>AgNO3;在三叶草愈伤组织的继代培养中,对愈伤组织褐化抑制效果较好的是AgNO3,浓度为0.015 g/L时,褐化率为32%,愈伤组织褐化程度强弱依次是柠檬酸>PVP>AgNO3。一定浓度的AgNO3不仅能有效控制褐化,还在一定程度上促进了愈伤组织的生长。

关键词: 山西中南部, 山西中南部, 区域性强降水, 流型配置, 物理量诊断

Abstract: To solve the browning in callus subculture of three legume forage species, callus ofMedicago sativa ‘Xinmu No.4’,Onobrychis viciaeflia andTrifolium repnes‘Haifa’were taken as test materials, the effect of different measures on preventing browning for the three legume forage species in callus subculture were discussed. The results showed that in callus subculture of M. sativa‘Xinmu No.4’and O. viciaeflia , theinhibition effect of 0.02 g/L AgNO3 was better and the browning rate was 13% and 15%, respectively, the browning degree of callus was PVP> citric acid> AgNO3; the inhibition effect of 0.015 g/L AgNO3 was better and the browning rate was 32% during callus subculture ofT. repnes‘Haifa’, the browning degree of callus was citric acid> PVP> AgNO3. A certain concentration of AgNO3 could inhibit browning as well as promote the growth of callus to some extent.