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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (8): 111-117.doi: 10.11924/j.issn.1000-6850.casb17090040

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

饲料添加白藜芦醇对吉富罗非鱼生长免疫、肝脏结构的影响

赵志祥,史磊磊,张 聪,郑 尧,陈家长   

  1. 南京农业大学无锡渔业学院,南京农业大学无锡渔业学院,中国水产科学研究院淡水渔业研究中心,中国水产科学研究院淡水渔业研究中心,中国水产科学研究院淡水渔业研究中心
  • 收稿日期:2017-09-08 修回日期:2018-02-20 接受日期:2017-12-20 出版日期:2018-03-21 发布日期:2018-03-21
  • 通讯作者: 陈家长
  • 基金资助:
    现代农业产业技术体系专项(CARS-49)。

Effects of Resveratrol on Growth Performance, Immunity and Liver Microstructure of GIFT (Oreochromis niloticus)

  • Received:2017-09-08 Revised:2018-02-20 Accepted:2017-12-20 Online:2018-03-21 Published:2018-03-21

摘要： (目的)为探究不同含量白藜芦醇对吉富罗非鱼(Oreochromis niloticus)生长、非特异免疫能力及肝脏结构的影响。(方法)试验设计4组不同含量Res饲料：0%、0.025%、0.05%、0.1%,分别表示D1、D2、D3、D4组；在第15天、45天测定血清中八种非特异免疫酶活性,扫描电镜观察肠绒毛、肝脏显微结构。(结果)结果显示：经45天,处理组与对照组之间增重率(WGR)、特定生长率(SGR)、饲料系数(FC)显著降低(P<0.05)。生长期15天,MT、Hsp-70、Hsp-60、TRF四种酶活性均随着Res含量提高而降低；C3、C4二种酶活性处理组与对照组无显著差异；生长期45天,C3、C4、IgM、TNF-α、Hsp-60、TRF六种酶活性均随着Res含量提高而降低。扫描电镜显示：15天D4组肠绒毛“干瘪”、“卷曲程度加深”,45天时,D4组绒毛排列紊乱、绒毛高度降低,受损严重,肝脏纤维化现象明显。(结论)综上所述：饲料中添加0.1% Res能够降低吉富罗非鱼部分非特异性免疫酶活性,并且损伤肠道、肝脏。

关键词: 毛乌素沙地东南缘, 毛乌素沙地东南缘, 寄主植物, 钻蛀性害虫, 名录

Abstract: Resveratrol has gained great interest due to various reported health benefits. However, little information is available about its effects on the genetically improved farmed tilapia (GIFT), Oreochromis niloticus. Hence, this study was conducted to investigate the effects of dietary supplemental resveratrol on the growth, non-specific immunity, intestinal villi and liver microstructure of GIFT. (Method) Fish were fed four diets with different levels of resveratrol: 0, 250, 500 and 1000mg/kg. Eight non-specific immune enzymes in serum were measured on the 45th day. (Result)The results showed that the weight gain rate (WGR), specific growth rate (SGR) and feed coefficient (FC) significantly decreased (P<0.05) with increment of resveratrol at average 6.5%, 4.0% and 8.3% respectively. Activities of C3, C4, IgM, TNF-α, Hsp-60 and TRF decreased with increasing addition of resveratrol. Furthermore, differentSdegrees of damage were observedSin intestinal villi and liver in treated groups. (Conclusion)Therefore, addition of 0.1% resveratrol in diets can partially decrease non-specific immune enzyme activities, and damage the intestine and liver of tilapia.