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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (9): 111-118.doi: 10.11924/j.issn.1000-6850.casb17100060

• 水产 渔业 • 上一篇    下一篇

锦鲤疱疹病毒病病原检测能力验证结果分析

张 旻,景宏丽,王 娜,吴绍强,李 清,余卫忠   

  1. 中国检验检疫科学研究院,中国检验检疫科学研究院,中国检验检疫科学研究院,中国检验检疫科学研究院,全国水产技术推广总站,全国水产技术推广总站
  • 收稿日期:2017-10-18 修回日期:2018-03-08 接受日期:2017-12-22 出版日期:2018-03-21 发布日期:2018-03-21
  • 通讯作者: 李 清
  • 基金资助:
    科技部“十二五”国家科技支撑计划“重大外来与新发水生动物疫病识别与监测技术研究及示范”(2013BAD12B02)。

The Proficiency Testing Results of Pathogen Detection Ability for Koi Herpesvirus Disease (KHVD)

  • Received:2017-10-18 Revised:2018-03-08 Accepted:2017-12-22 Online:2018-03-21 Published:2018-03-21

摘要： 为了提高中国锦鲤疱疹病毒病(KHVD)病原检测的整体水平，加强相关实验室检测锦鲤疱疹病毒(KHV)的能力，2014—2016年开展了3次能力验证项目。能力验证样品为盲样，使用鲤鱼组织匀浆液，与包含了KHV TK基因片段和Sph基因片段的2种重组质粒溶液混合制成。对盲样进行了均匀性和稳定性检测。采用水产行业标准《鲤疱疹病毒检测方法 第1部分：锦鲤疱疹病毒》(SC/T 7212.1-2011)中，推荐使用的聚合酶链式反应(PCR)方法进行病毒检测，并对各参加单位锦鲤疱疹病毒的检测结果进行分析。2014年能力验证第一轮测试结果满意率为58%，第二轮满意率为96%；2015年能力验证第一轮测试结果满意率为77%，第二轮满意率为89%；2016年能力验证第一轮测试结果满意率为82.69%，第二轮满意率为92.73%。通过2014至2016年的能力验证活动，大多实验室具备了锦鲤疱疹病毒PCR检测能力和质量管理水平，可以承担锦鲤疱疹病毒的检测和监测工作。

Abstract: To improve the overall level of pathogen detection of Koi herpesvirus disease (KHVD) in China, and strengthen the detection ability of laboratories for Koi herpesvirus (KHV), the proficiency testing programs were carried out for 3 times from 2014 to 2016. Blind samples were used in the proficiency testing programs, which were prepared by mixing carp tissue homogenate with recombinant plasmids containing KHVTK gene fragment and Sph gene fragment. The homogeneity and stability of the blind samples were examined. Polymerase Chain Reaction (PCR) assay, which was recommended by the aquaculture industry standard, Detection Methods of Herpesvirus (CyHv)-Part I: Koi herpevirus (SC/T 7212.1-2011), was used to detect KHV, and the test results of all participating laboratories were analyzed. The satisfaction rate of the first round test results was 58% in 2014, after the second round test, the overall satisfaction rate was 96%. In 2015, the satisfaction rate of the first round test results was 77%, the overall satisfaction rate was 89% after the second round tests. The satisfaction rate of the first round tests results was 82.69% in 2016, and the overall satisfaction rate was 92.73% after the second round tests. Through the proficiency testing programs of 2014-2016, most attended laboratories possessed the ability of PCR detection of Koi herpesvirus and the capacity of quality management, and could undertake the detection and monitoring of Koi herpesvirus.