黑腹果蝇和斑翅果蝇肠道可培养的细菌多样性分析

doi:10.11924/j.issn.1000-6850.casb17100064

中国农学通报 ›› 2019, Vol. 35 ›› Issue (9): 61-67.doi: 10.11924/j.issn.1000-6850.casb17100064

黑腹果蝇和斑翅果蝇肠道可培养的细菌多样性分析

高欢欢¹, 吕召云², 王咏梅¹, 蒋锡龙¹, 刘倩², 吴新颖¹, 于毅²

1.山东省葡萄研究院;2.山东农业科学院植物保护研究所

收稿日期:2017-10-19 修回日期:2019-03-15 接受日期:2018-01-24 出版日期:2019-03-26 发布日期:2019-03-26
通讯作者: 高欢欢
基金资助:
国家自然科学基金青年科学基金项目(31801750)；山东省现代农业产业技术体系果品创新团队济南综合试验站(SDAIT-06-21)；山东省农业科学院农业科技创新工程(CXGC2018E17)。

Diversity Analysis of Culturable Bacteria in Intestine of Drosophila melanogaster and Drosophila suzukii

Received:2017-10-19 Revised:2019-03-15 Accepted:2018-01-24 Online:2019-03-26 Published:2019-03-26

摘要/Abstract

摘要： 为了了解斑翅果蝇Drosophila suzukii和黑腹果蝇D. melanogaster可培养的肠道细菌多样性。本研究将2种果蝇卵中及幼虫、蛹和成虫肠道中的细菌分别进行分离纯化，并利用16srDNA基因分析的方法鉴定。结果显示，在2种果蝇的不同发育阶段中，卵中的细菌数量最少，而幼虫肠道中最多，且斑翅果蝇中细菌的数量显著多于黑腹果蝇。2种果蝇共分离出31种细菌，以变形菌门细菌为主。斑翅果蝇和黑腹果蝇中细菌的种类分别为20和21种，其中相同种类的细菌为13种。斑翅果蝇肠道内比较稳定的细菌种类为弗氏柠檬酸杆菌Citrobacter freundii、产酸克雷伯菌Klebsiella oxytoca、金黄杆菌属Chryseobacterium sp.、形赖氨酸芽孢杆菌Lysinibacillus fusiformis，黑腹果蝇肠道内比较稳定的细菌种类为醋酸菌Acetobacter thailandicus、类芽孢杆菌Paenibacillus taichungensis、雷氏普罗威登斯Providencia rettgeri、摩氏摩根菌Morganella morganii。因此，不同发育阶段的斑翅果蝇和黑腹果蝇肠道中细菌的数量和种类均有差别。

Abstract: To understand the intestinal bacterial diversity in Drosophila suzukii and D. melanogaster, the bacteria in eggs, the intestine of larvae, pupae and adults of two Drosophila species were separated and purified, and identified with 16S rDNA genetic analysis. The results showed that, the number of bacteria was the lowest in eggs and the highest in larval intestine at different developmental stages of the two Drosophila species. And the number of bacteria in D. suzukii was more than that in D. melanogaster ignificantly. 31 species of bacteria were isolated from the two Drosophila species, the bacteria of Proteobacteria was the dominant population. The species of bacteria were 20 and 21 in D. suzukii and D. melanogaster, respectively. Therein, there were 13 species in both the two Drosophila species. The stable bacteria in intestine of D. suzukii were Citrobacter freundii, Klebsiella oxytoca, Chryseobacterium sp. and Lysinibacillus fusiformis. While, Acetobacter thailandicus, Paenibacillus taichungensis, Providencia rettgeri and Morganella morganii were stable bacteria in intestine of D. melanogaster. It is concluded that, the number and species of bacteria in D. suzukii and D. melanogaster are different at different development stages.

高欢欢,吕召云,王咏梅,蒋锡龙,刘倩,吴新颖,于毅. 黑腹果蝇和斑翅果蝇肠道可培养的细菌多样性分析[J]. 中国农学通报, 2019, 35(9): 61-67.

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黑腹果蝇和斑翅果蝇肠道可培养的细菌多样性分析

Diversity Analysis of Culturable Bacteria in Intestine of Drosophila melanogaster and Drosophila suzukii

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