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中国农学通报 ›› 2018, Vol. 34 ›› Issue (24): 71-76.doi: 10.11924/j.issn.1000-6850.casb17110016

• 食品 营养 检测 安全 • 上一篇    下一篇

奶粉加工环节阪崎克罗诺杆菌的分离鉴定及同源性分析

郝悦,刘峰,雷虹,孟利   

  1. 黑龙江大学 黑龙江省普通高等学校分子生物学重点实验室 黑龙江 哈尔滨,黑龙江省农业科学院农产品质量安全研究所,黑龙江大学 黑龙江省普通高等学校分子生物学重点实验室 黑龙江 哈尔滨,黑龙江大学 黑龙江省普通高等学校分子生物学重点实验室 黑龙江 哈尔滨
  • 收稿日期:2017-11-07 修回日期:2018-08-03 接受日期:2018-01-24 出版日期:2018-08-28 发布日期:2018-08-28
  • 通讯作者: 孟利
  • 基金资助:
    黑龙江省普通高等学校分子生物学重点实验室(黑龙江大学)开放课题“婴儿奶粉生产线阪崎阪肠杆菌污染溯源分析及控制”(1201)。

Separation, Identification and Homology Analysis of Cronobacter sakazakii in Milk Powder Processing

  • Received:2017-11-07 Revised:2018-08-03 Accepted:2018-01-24 Online:2018-08-28 Published:2018-08-28

摘要: 分析奶粉厂可能被阪崎克罗诺杆菌污染的环节,及被检出阪崎克罗诺杆菌的同源性,旨在有效降低奶粉厂阪崎克罗诺杆菌污染风险。利用生理生化法、PCR法和16SrDNA序列分析法分析在配料、生产线、设备及环境收集的微生物样品。共采样25处,其中8处有菌检出,3株鉴定为阪崎克罗诺杆菌。利用16srDNA法对阪崎克罗诺杆菌(ATCC 29544)C.sakazakii E、问题奶粉中的C.sakazakii A、回收细粉中的C.sakazakii H、准备间地漏中的C.sakazakii D四株进行同源性分析,结果表明C.sakazakii A与C.sakazakii H的同源性最高,说明成品中阪崎肠杆菌污染可能源于回收细粉。将8处有菌检出的环节设为关键控制点,其中回收细粉处为最为关键环节,与准备间地漏一同作为阪崎克罗诺杆菌危害关键控制点,本研究对奶粉厂阪崎克罗诺杆菌的控制提供了数据依据。

Abstract: To reduce the risk of Cronobacter sakazakii contamination effectively,samples from milk power factory manufacturing units which may be contaminated by the C. sakazakii were detected and the homology of C. sakazakii found in the sampls was determined. Physiological biochemical method, PCR method and 16SrDNA sequence analytical method were applied to analyze the microbial samples from ingredients, production line, equipment and environment. In 25 samples collected, bacteria were found in 8 samples of which 3 were identified to be C. sakazakii. Homology of C.sakazakii. E(ATCC 29544), C. sakazakii A found in milk powder, C. sakazakii H found in recovery fine powder and C. sakazakii D from the floor drain were analyzed by the 16SrDNA gene sequence, the results showed that C. sakazakii A had the highest homology with C. sakazakii H.The C. sakazakii contamination in the power products may come from the recovery of fine powder. The 8 points where bacteria were found were set to be key control points and recovery fine powder was the most critical control point. The recovery fine power and the floor drain were critical control points for Cronobacter sakazakii control. The study provides data for Cronobacter sakazakii control in milk power factory.