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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (9): 143-148.doi: 10.11924/j.issn.1000-6850.casb17110081

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

六种杀菌剂对黄瓜尖孢镰刀菌的毒力测定试验

王芳   

  1. 广东佛山科学技术学院食品科学与工程学院
  • 收稿日期:2017-11-21 修回日期:2019-03-18 接受日期:2018-02-24 出版日期:2019-03-26 发布日期:2019-03-26
  • 通讯作者: 王芳
  • 基金资助:
    国家级大学生创新创业训练计划项目“蔬菜几种主要真菌病害的药剂筛选”(XJ2016038)。

Toxicity Test of Six Fungicides on Fusarium Oxysporum Infecting Cucumber

  • Received:2017-11-21 Revised:2019-03-18 Accepted:2018-02-24 Online:2019-03-26 Published:2019-03-26

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摘要： 本试验分离与纯化黄瓜枯萎病病原菌，鉴定出病原菌为尖孢镰刀菌（Fusarium oxysporumSchl.）。采用含毒培养基生长速率法测定六种杀菌剂的抑制效果，以菌落直径为实验观察指标，测量对照组和处理组的菌落直径，以抑制率测定杀菌剂对尖孢镰刀菌的抑制效果。结果表明：药后72h，杀菌剂丙环唑的EC50值为0.9039，其毒力指数最大，对尖孢镰刀菌的抑制效果最佳；代森、异菌肽的EC50值为2.8939、6.8186，效果较好；醚菌酯、嘧霉胺的EC50值为29.3951、30.4126，效果一般；苯醚甲环唑的EC50值为143.2943，毒力指数最小，抑制效果较差。

Abstract: The aim of this studyis to identify the inhibitory effects of six fungicides by the growth rate method on toxic culture medium. The colony diameterwasused as observation index. The colony diameters of the control group and the treatment group were detected, and the inhibitory effects offungicides on Fusarium Oxysporum were measured by inhibition ratio. The results showed that72 hours after medication, propiconazole had the maximum toxicity index and the best inhibition effect onFusarium oxysporum with EC50 value of 0.9039 mg/L, followed by mancozeb and isopeptide, with the EC50 value of 2.8939 mg/L and 6.8186 mg/L, respectively. Kresoxim- methyl and pyrimethanil had normal effects,with EC50 value of 29.3951 mg/L and 30.4126 mg/L, respectively. Difenoconazole had the minimumtoxicity index and the least effect, with EC50 value of 143.2943 mg/L. In conclusion, propiconazole hasthe best inhibition effect.