红富士苹果花挥发性成分分析

doi:10.11924/j.issn.1000-6850.casb18010070

中国农学通报 ›› 2018, Vol. 34 ›› Issue (15): 60-65.doi: 10.11924/j.issn.1000-6850.casb18010070

所属专题：园艺

红富士苹果花挥发性成分分析

马卫华,李磊,武文卿,宋怀磊,李立新,申晋山

山西农业科学院园艺研究所,山西农业科学院园艺研究所,山西农业科学院园艺研究所,山西农业科学院园艺研究所,山西农业科学院园艺研究所,山西农业科学院园艺研究所

收稿日期:2018-01-12 修回日期:2018-05-08 接受日期:2018-03-23 出版日期:2018-05-29 发布日期:2018-05-29
通讯作者: 申晋山
基金资助:
现代农业产业技术（蜜蜂）体系建设(CARS-44-KXJ23)；山西省农业科学院创新课题(YCX2017D2306)。

Flowers of Fuji Apple: Volatile Components Analysis

Received:2018-01-12 Revised:2018-05-08 Accepted:2018-03-23 Online:2018-05-29 Published:2018-05-29

摘要/Abstract

摘要： 为了了解红富士苹果花散粉后的挥发性物质，探讨其挥发性物质和传粉昆虫的关系，为苹果蜜蜂授粉提供思路。笔者采用固相微萃取和气相色谱-质谱联用技术，测定红富士苹果花散粉后的挥发性物质，进行定性分析；按面积归一化法进行定量分析。结果表明：从红富士苹果鲜花中共检测鉴定出31种挥发性成分，有醇类4种(76.05%)、芳香烃类4种(5.36%)、腈类1种（橙花腈，4.6%）、烷烃类10种(4.54%)、酯类7种(3.86%)、烯类3种(3.71%)、醛类1种（壬醛，1.67%）、酸类1种（月桂酸，0.21%）组成。醇类中以苯甲醇含量高，为49.74%；其次芳樟醇17.9%，(Z)-3-己烯-1-醇7.82%，雪松醇0.59%。烯类有罗勒烯、石竹烯和法呢烯，罗勒烯含量最高为2.69%。苯甲醇、(Z)-顺-3-己烯-1-醇、芳樟醇、橙花腈为苹果鲜花挥发物的主体成分。

关键词: 鸡MHCⅠ分子, 鸡MHCⅠ分子, 二聚体, 真核表达

Abstract: The paper aims to discuss the relationship between volatile components and pollination insects by learning about volatile components of Fuji apple flowers, and to provide a research basis for bee pollination on apple. Headspace solid-phase microextraction (HS-SPME) combined with gas chromatographymass spectrometry (GC-MS) was applied to measure and analyze the volatile components of live flowers after anther dehiscence during apple blossom; and a quantitative analysis was conducted with the method of area normalization. The results showed that: 31 kinds of volatile components were identified from Fuji apple flowers, including 4 kinds of alcohols (76.05%), 4 kinds of aromatic hydrocarbons (5.36%), 1 kind of nitrile (neryl nitrile, 4.6%), 10 kinds of alkanes (4.54%), 7 kinds of esters (3.86%), 3 kinds of terpene (3.71%), 1 kind of aldehydes (nonanal, 1.67% ), 1 kinds of acids (Dodecanoic acid, 0.21% ). Benzyl alcohol had the highest content in alcohols, accounted for 49.74%, followed by linalool (17.9%), (Z )-3-Hexen-1-ol (7.82%) and cedrol (0.59%). Terpene components included 1,3,6- Octatriene, 3,7- dimethyl- ,(Z ), caryophyllene and alpha.- farnesene, and the content of 1,3,6-Octatriene, 3,7-dimethyl-,(Z ) (2.69%) was the highest in terpene. Benzyl alcohol, (Z )-cis-3-hexene-1-alcohol, linalool, neryl nitrile were the main volatile components in Fuji apple flowers.

马卫华,李磊,武文卿,宋怀磊,李立新,申晋山. 红富士苹果花挥发性成分分析[J]. 中国农学通报, 2018, 34(15): 60-65.

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红富士苹果花挥发性成分分析

Flowers of Fuji Apple: Volatile Components Analysis

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