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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (14): 41-46.doi: 10.11924/j.issn.1000-6850.casb18020067

• 林学 园艺 园林 • 上一篇    下一篇

绒毛白蜡组培快繁研究

王因花1, 燕丽萍1, 孔雨光2, 李丽1, 王开芳1, 刘翠兰1, 任飞1, 吴德军1   

  1. 1.山东省林业科学研究院;2.山东省林业监测规划院
  • 收稿日期:2018-02-23 修回日期:2018-12-04 接受日期:2018-12-07 出版日期:2019-05-15 发布日期:2019-05-15
  • 通讯作者: 燕丽萍
  • 基金资助:
    山东省科技发展计划“白蜡种质资源分子评价与体胚发育技术研究”(2014GNC111006)；国家“十二五”科技支撑计划“白蜡种质资源发掘 与创新利用”(2013BAD01B06-6)。

Tissue Culture of Fraxinus velutina: Rapid Propagation

  • Received:2018-02-23 Revised:2018-12-04 Accepted:2018-12-07 Online:2019-05-15 Published:2019-05-15

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摘要： 为探索建立绒毛白蜡组织培养快速繁育体系，本研究以绒毛白蜡带芽茎段为材料，探讨了基本培养基、植物生长调节剂浓度及配比对绒毛白蜡组培快繁效果的影响。结果表明，MS、DKW均可作为绒毛白蜡繁殖的基本培养基，最佳增殖培养基为MS+0.5 mg/L TDZ+0.5 mg/L 6-BA+0.5 mg/L IBA，平均增殖系数达7.1，最佳壮苗培养基为MS+0.1 mg/L KT+0.5 mg/L TDZ+0.01 mg/L NAA，平均苗高达7.15 cm。

关键词: 植物U-box蛋白, 植物U-box蛋白, 先天免疫反应, E3泛素连接酶, 病原相关分子激发的免疫反应, 效应蛋白激发的免疫反应

Abstract: The paper aims to establish a rapid breeding system of Fraxinus velutina. Taking the stem segment with axillary buds as materials, we explored the effects of basic medium, plant growth regulator concentrations and ratios on the rapid propagation for tissue culture of Fraxinus velutina. The results showed that: MS and DKW could both be used as the basic medium for the tissue culture of Fraxinus velutina; the best multiplication culture medium was MS+ 0.5 mg/L TDZ+0.5 mg/L 6-BA+0.5 mg/L IBA with the average coefficient of multiplication of 7.1, and the optimal seedling medium was MS+0.1 mg/L KT+0.5 mg/L TDZ+ 0.01 mg/L NAA and the average seedling height was 7.15 cm